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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 33 results for: vent

Showing: 1 - 20 of 33 results

  1. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...separate DNA molecules (in trans), a translocation event is generated between the target sites. Created with...cassette) placed upstream of a gene of interest will prevent gene expression in the absence of Cre. In the presence...combined in multiple, independent recombination events in a single experimental system, enabling multi-layered... the target site cassette in a double-crossover event. Toxicity Cre-lox recombination is a very useful...lineage-specific drivers can also help reduce off-target events and the systemic effects of Cre toxicity. Leakiness...iCre variant, reduces the number of recombination events but can improve specificity in targeted neuronal...

  2. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...hU6 none S. pyogenes Ventura pDonor_mU6 69350 Mammalian mU6 none S. pyogenes Ventura pDonor_sU6 69351 Mammalian...64073 Mammalian BbsI and BsaI yes, cut S. pyogenes Ventura PX851 62883 Mammalian BbsI yes, cut, N-terminal...Mammalian sU6 none S. pyogenes Ventura pCRISPRyl 70007 Other/Yarrowia lipolytica SCR1'-tRNA yes, cut S...independent U6 promoters and Cas9 from CBh promoter. Ventura A CRISPR/Cas9 toolkit for multiplex genome editing...

  3. Brain Initiative Collection

    Type
    Collection
    ...will provide novel ways to treat, cure, and even prevent brain disorders. The BRAIN Initiative supports ... coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f. Edward Boyden 158757-AAV9 pAAV-CAG-... coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f. Edward Boyden 163505-PHPeB CN1390-rAAV-DLX2.0...

  4. Serotype Testing AAV

    Type
    Collection
    ...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list ...100 µL sized sample, please see our Control AAV Inventory . The catalog ID for the 20 µL sized testing sample...

  5. CRISPR Guide

    Type
    Collection
    ...RuvC and HNH, can be altered to reduce cleavage events to a single cut (nick) or no cuts. Cas9 nickase...the donor template, incorporating mutations that prevent further Cas9 cleavage will improve the accuracy...and NGS (other genomic regions). Compared to conventional methods for genomic purification, CRISPR-based...Rekhtman, N., De Stanchina, E., Lowe, S. W., & Ventura, A. (2014). In vivo engineering of oncogenic chromosomal...

  6. Neurodegeneration Research Collection

    Type
    Collection
    ...understood, and there are no effective therapies to prevent, treat, or cure most of these disorders. An integrated...age of 60. There is presently no cure or way to prevent progression of PD. Treatment consists mainly of...

  7. CRISPR Plasmids - Tagging

    Type
    Collection
    ...principle of the PITCh system is different from the conventional HDR-mediated gene knock-in. The detailed procedure...and a repair template for an individual tagging event. The kit contains 26 plasmids; 21 of the plasmids...

  8. Bacterial Expression Systems

    Type
    Collection
    ...Plasmids Reporter plasmids can be used to detect events and molecules in and around a bacterium. Plasmids...genetic inversion, deletions, and translocations events. Visit our TALEN Collection page to find plasmids...

  9. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...using various delivery methods including intracerebroventricular (ICV) delivery in newborns, and retro-orbital...minBG-SYFP2-WPRE3-BGHpA AiP14470 AiE0027m_3xC3 SYFP2 Ventromedial hypothalamic nucleus (VMH) Hypothalamus 230531...

  10. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling. J Cell Biol. 2010 Feb...Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage...

  12. Chemogenetics Plasmids

    Type
    Collection
    ...chemogenetic resources available at Addgene. See our inventory of chemogenetic constructs, including DREADD constructs...

  13. Mammalian RNAi Tools

    Type
    Collection
    ...shRNA design in pSico with pSicoligomaker (Andrea Ventura lab) References Carthew, R. W., & Sontheimer, E...

  14. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...critical for developing therapeutic strategies to prevent viral spread. As a Biosafety Level 3 (BSL-3) agent...

  16. Recombinases AAV Preps

    Type
    Collection
    ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ...

  17. Chemogenetics AAV Preps

    Type
    Collection
    ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t See ...

  18. Viral Vector Guides and Plasmids

    Type
    Collection
    ...Specific genes have been mutated or deleted to prevent uncontrolled viral replication, and the required...

  19. Antibody Plasmid Collection

    Type
    Collection
    ...dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs...

  20. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...additional protection added to 3’ tail of pegRNA to prevent RNA degradation For more in-depth information on...
Showing: 1 - 20 of 33 results