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Plasmid#223008PurposeControl plasmid without Cas and Csy4 for Nicotiana leaf assayDepositorInsertNot applicable, control plasmid
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB26
Plasmid#223009PurposeCas12a and Csy4 with 5' target (I) for Nicotiana leaf assayDepositorInsertCas12a and Csy4 with 5' target (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB27
Plasmid#223010PurposeCas12a and Csy4 with 3' target (I) for Nicotiana leaf assayDepositorInsertCas12a and Csy4 with 3' target (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB28
Plasmid#223011PurposeCas12a and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assayDepositorInsertCas12a and Csy4 with 5' and 3' targets (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB61
Plasmid#223014PurposeCas12a and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assayDepositorInsertCas12a and Csy4 with 5' and 3' targets (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB105
Plasmid#223016PurposeCas9 and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assayDepositorInsertCas9 and Csy4 with 5' and 3' targets (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0194 CMVTO-secNLuc-rens-mem
Plasmid#231211PurposeMembrane-bound nanoluciferaseDepositorInsertsecNLuc-rens-mem
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL661-TTTA
Plasmid#215865PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL661 with TTTA stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL714-TTTA
Plasmid#215867PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL714 with TTTA stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL864-TTTA
Plasmid#215859PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL864 with TTTA stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL864R-TTTA
Plasmid#215861PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL864R with TTTA stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL554-TTTA
Plasmid#215863PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL554 with TTTA stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FB367
Plasmid#203630PurposeModule for the expression of geneticin resistance and Nanoluciferase.DepositorInsertPgpdA:Nluc:Ttub::PtrpC:nptII:Ttub
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAP233
Plasmid#137753PurposeExtracellular control for HiBiTopt experimentsDepositorInsertsrtB-linker-hibitopt
UseLuciferaseTagsHiBiToptExpressionBacterialMutationPromoterPtetAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH741-CENslow-RLuc
Plasmid#38221DepositorInsertRenilla Luciferase
UseTagsExpressionYeastMutationPromoterADH1Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Control-miR-17-5p-Control
Plasmid#21167DepositorInsertmiR 17-5p Sensor (MIR17 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
CDS_LUC
Plasmid#136083PurposeFirefly luciferaseDepositorInsertCDS_LUC
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-minP:luc2
Plasmid#138354PurposeGateway compatible middle entry clone contains Firefly Luciferase2 insert driven by minimal promoterDepositorInsertLuciferase
UseTagsExpressionMammalianMutationPromoterminimal promoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialMutationPromoterPydfJ115Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUAS-NanoLuc
Plasmid#87696PurposeGal4VP16 driven expression of NanoLucDepositorInsertNanoLuc
UseTagsnuclear localization signalExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX304_CMV::mCherry-LgBiT-FLAG-NES
Plasmid#199715PurposeExpresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.DepositorInsertmCherry-LgBiT-FLAG-NES
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pluc2-iRFP720
Plasmid#98887PurposeMammalian expressionDepositorInsertFirefly Luciferase
UseTagsiRFP720ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC0051-W85X REPAIR targeting guide cloned into pC0043
Plasmid#103867PurposeGuide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038DepositorInsertW85X targeting guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP24
Plasmid#70190PurposeModular vector for secreted luciferase reporter expressionDepositorInsertsLucopt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK-hFluc-CrPV-hRluc-6xbsMS2-Malat_AC
Plasmid#148443PurposeMammalian Expression of 6xbsMS2-MalatDepositorInsert6xbsMS2-Malat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA nLuc SV40
Plasmid#132654PurposeExpresses nanoLuciferase (nLuc) mRNA from the Drosophila Metallothionein A promoterDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-T-Lg
Plasmid#216977PurposeExpression of GvpT-Linker-Large BiTDepositorInsertGvpT-Linker-Large BiT
UseTagsSplit Nanoluciferase LargeBiT-FLAGExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-Sm-U
Plasmid#217003PurposeExpression of Small BiT-Linker-GvpUDepositorInsertSmall BiT-Linker-GvpU
UseTagsSplit Nanoluciferase SmallBiTExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-G-Sm
Plasmid#216990PurposeExpression of GvpG-Linker-Small BiTDepositorInsertGvpG-Linker-Small BiT
UseTagsSplit Nanoluciferase SmallBiTExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-N-Lg
Plasmid#216963PurposeExpression of GvpN-Linker-Large BiTDepositorInsertGvpN-Linker-Large BiT
UseTagsSplit Nanoluciferase LargeBiT-FLAGExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only