We narrowed to 10,941 results for: NSI;

1. pCS2 Flag-JunB

   Plasmid

   #29687

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   jun B oncogene (JUNB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAS1NB c Rosella I

   Plasmid

   #71245

   Purpose

   a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

   Depositor

   Mark Prescott

   Article

   Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

   Insert

   Rosella
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK1

   Available since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p3xFLAG-CMV-14-GINIP-Nluc

   Plasmid

   #223556

   Purpose

   Plasmid expressing GINIP-Nluc in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

   Insert

   human GINIP - linker(GGGS) - Nluc
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-TRE-DIO-hM3Dq-mCherry

   Plasmid

   #166599

   Purpose

   Encodes Cre-dependent hM3Dq-mCherry under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTK5

   Plasmid

   #37391

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

   Insert

   ACTR1A (ACTR1A Human)
   

   Use

   Tags

   S-tag, TEV, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-SisTnpB1–ωRNA

   Plasmid

   #228365

   Purpose

   Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

   Depositor

   Tao Liu

   Article

   Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

   Insert

   SisTnpB1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-PKCA WT

   Plasmid

   #195202

   Purpose

   Mammalian expression vector for GFP tagged PKCA.

   Depositor

   Peter Parker, David Bryant

   Article

   Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

   Insert

   PRKCA (PRKCA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBLADE_ONLY_C

   Plasmid

   #168050

   Purpose

   Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

   Depositor

   Barbara Di Ventura

   Article

   Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

   Insert

   Blue light-inducible AraC dimers in Escherichia coli
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101**

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gi)

   Plasmid

   #230019

   Purpose

   AAV vector expressing anti-mCherry PAGER(Gi) in neurons

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-LaM6-TEVcs-ALFA-hM1D
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Clomeleon

    Plasmid

    #203723

    Purpose

    A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

    Depositor

    George Augustine

    Article

    Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

    Insert

    cyan fluorescent protein linked yellow florescent protein
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Cyan Fluorescent Protein, Topaz Fluorescent Prote…

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNTI661 pRPR1(TetO)-sgRNA

    Plasmid

    #139475

    Purpose

    Vector for tet-regulated sgRNA expression in yeast

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Insert

    single guide RNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pRPR1(TetO)

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCITF-KCC2

    Plasmid

    #61404

    Purpose

    Mammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequence

    Depositor

    Franck Polleux

    Article

    Bortone et al Neuron. 2009 Apr 16;62(1):53-71. doi: 10

    Insert

    KCC2 (SLC12A5 Human)
    

    Use

    Tags

    IRES-tdTomato-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBLADE_ONLY_A

    Plasmid

    #168051

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Insert

    Blue light-inducible AraC dimers in Escherichia coli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101**

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMN-332

    Plasmid

    #195466

    Purpose

    Lentiviral Integratable Inducible synZiFTR ZF1 + Reporter

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    inverted[8X ZF1 BS - ybTATA - mCherry] - pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CAG-miniGFP1-P2A-FusionRed

    Plasmid

    #188732

    Purpose

    Express miniGFP1 and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP1
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQAT59

    Plasmid

    #223431

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-anti-mCherry(LaM6) PAGER(Gs)

    Plasmid

    #230011

    Purpose

    Expresses anti-mCherry PAGER(Gs) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM1Dxs
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FUGW-VGLUT2-pHluorin

    Plasmid

    #207469

    Purpose

    Lentiviral expression of VGLUT2-pHluorin

    Depositor

    Robert Edwards

    Article

    Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

    Insert

    VGLUT2 (Slc17a6 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDae069 (eMutaT7[PmCDA1] with optimized RBS)

    Plasmid

    #187621

    Purpose

    Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Insert

    PmCDA1-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiCRISPR V2 sgIREB2_2

    Plasmid

    #169886

    Purpose

    Disrupt IREB2

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    sgRNA targeting IREB2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMSCVhyg-3xT7-CA-ChREBP

    Plasmid

    #171029

    Purpose

    Expresses 3xT7-tagged constitutive active form of ChREBP in mammalian cells

    Depositor

    Kai Ge

    Article

    Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

    Insert

    CA-ChREBP (Mlxipl Mouse)
    

    Use

    Retroviral

    Tags

    3xT7

    Expression

    Mammalian

    Mutation

    amino acids 240 - 864

    Promoter

    5' LTR

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCSDest-HA-TMPRSS2

    Plasmid

    #154963

    Purpose

    TMPRSS2 with N-terminal HA

    Depositor

    Utpal Pajvani

    Article

    Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

    Insert

    HA-TMPRSS2 (TMPRSS2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pLentiCRISPRv2 Neo

    Plasmid

    #127644

    Purpose

    Empty pLentiCRISPRv2 with NeoR

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-NP-V5-B/BRISBANE/60/2008

    Plasmid

    #187923

    Purpose

    Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    Influenza B Nucleoprotein
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-CAG-miniGFP2-P2A-FusionRed

    Plasmid

    #188734

    Purpose

    Express miniGFP2 and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP2
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. H2B-pDendra2(N)

    Plasmid

    #75283

    Purpose

    Expresses H2B-Dendra2 in mammalian cells

    Depositor

    Xavier Darzacq

    Article

    Recamier et al Nucleus. 2014 Jan-Feb;5(1):75-84. doi: 1

    Insert

    H2bk (H2BC12 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFastBac_human-IL-18

    Plasmid

    #214338

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pARF6-CFP

    Plasmid

    #11382

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 6 (ARF6 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-anti-GFP(LaG16) PAGER(Gi)

    Plasmid

    #230008

    Purpose

    Expresses anti-GFP PAGER(Gi) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaG16-TEVcs-ALFA-hM4D
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX330_human CDK1

    Plasmid

    #118597

    Purpose

    One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    guide RNA against human CDK1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. KillerOrange /pQE-30

    Plasmid

    #74748

    Purpose

    KillerOrange gene for bacterial expression

    Depositor

    Konstantin Lukyanov

    Article

    Sarkisyan et al PLoS One. 2015 Dec 17;10(12):e0145287. d

    Insert

    KillerOrange
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    KillerRed with substitutions G3C Y66W D113S N145S…

    Promoter

    T5

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(FL)

    Plasmid

    #230024

    Purpose

    Expresses anti-TNFalpha PAGER(FL) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    pAAV-MT3-Ozoralizumab-TEVcs-ALFA-hM4DxcpGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAV-U6+27-Tornado-Squash-SAM-sensor 5-1

    Plasmid

    #177917

    Purpose

    Expresses Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-Squash-SAM-sensor 5-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MAC-tag-C

    Plasmid

    #108077

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/BioID

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CCDB (ccdB(letD) )
    

    Use

    Tags

    StrepIII-HA-BirA*

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Integrin alpha5-AHCys

    Plasmid

    #27313

    Depositor

    Timothy Springer

    Article

    Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

    Insert

    integrin alpha 5 (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    integrin alpha5 (1-954) + AHCys

    Promoter

    Available since

    March 8, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pWM_12x601_35bpLinker

    Plasmid

    #157790

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_35bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. human PNP non-tagged

    Plasmid

    #64199

    Purpose

    Expression of native human PNP (non-tagged)

    Depositor

    Vern Schramm

    Article

    Murkin et al Biochemistry. 2007 May 1;46(17):5038-49.

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAID1.2-EF1a-NmScarlet-mAID

    Plasmid

    #140617

    Purpose

    Expression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAGGS-ChrimsonR-mRuby2-ST

    Plasmid

    #105447

    Purpose

    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

    Insert

    ChrimsonR-mRuby2-ST
    

    Use

    Tags

    mRuby2 and soma targeting motif

    Expression

    Mammalian

    Mutation

    K176R in Chrimson

    Promoter

    pCAGGS

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV-TetOn-NLS-mCherry

    Plasmid

    #193762

    Purpose

    Doxycycline-inducible (TetOn) mCherry with SV40 NLS, control construct

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    NLS-mCherry
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    SV40 NLS fused to mCherry

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge