Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 8,863 results for: BLI;

Showing: 5151 - 5200 of 8863 results

  1. mu-IFNg-pCIneo

    Plasmid
    #163517
    Purpose
    Expresses murine IFN gamma in mammalian cells
    Depositor
    Thomas Weber
    Insert
    murine-IFN gamma (Ifng Mouse)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163517-plasmid-map-sequence-id-321578

    Enlarge

  3. MSCV-Jarid1c-IRES-GFP

    Plasmid
    #166009
    Purpose
    Retroviral biscistronic expression of Jarid1c and eGFP
    Depositor
    Manu Rangachari
    Insert
    Jarid1c (Kdm5c Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166009-plasmid-map-sequence-id-327808

    Enlarge

  4. pcDNA-mC/EBPb

    Plasmid
    #49198
    Purpose
    expression of mouse C/EBPbeta in mammalian cells
    Depositor
    Jed Friedman
    Insert
    C/EBPbeta (Cebpb Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    49198-plasmid-map-sequence-id-265902

    Enlarge

  6. ADA

    Plasmid
    #39020
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADA (ADA Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. GFP-S100A11

    Plasmid
    #107201
    Purpose
    Expresses GFP-human S100A11 fusion protein for live cell imaging
    Depositor
    Ursula Rescher, Volker Gerke
    Insert
    S100A11 (S100A11 Human)
    Use
    Tags
    Green Fluorescent Protein
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107201-plasmid-map-sequence-id-209813

    Enlarge

  8. pNtCp-rbcL-PHLS-GPPS-aadA-accD

    Plasmid
    #52471
    Purpose
    Inserts the beta-phellandrene synthase gene, the geranyl diphosphate synthase gene, and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum
    Depositor
    Anastasios Melis
    Insert
    rbcL-PHLS-GPPS-aadA-accD
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52471-plasmid-map-sequence-id-81334

    Enlarge

  9. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51085
    Purpose
    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51085-plasmid-map-sequence-id-148706

    Enlarge

  10. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid
    #70684
    Purpose
    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.
    Depositor
    Bob Goldstein
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    70684-plasmid-map-sequence-id-306412

    Enlarge

  11. Human-Dysferlin-Original

    Plasmid
    #60216
    Purpose
    Human dysferlin (accession #NM_003494) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    60216-plasmid-map-sequence-id-96330

    Enlarge

  12. KLHL12

    Plasmid
    #38908
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KLHL12 (KLHL12 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. Aiolos ZF2 (AA146-168) in Cilantro 2

    Plasmid
    #74456
    Purpose
    Monitor post-translational degradation of Aiolos ZF2 via EGFP:mCherry ratio (Lentiviral, PGK.AiolosAA146-168-10AAFlexLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Insert
    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    74456-plasmid-map-sequence-id-140672

    Enlarge

  14. meGFP-hTRAK1

    Plasmid
    #188664
    Purpose
    human TRAK1 with N-terminal meGFP tag
    Depositor
    Thomas Schwarz
    Insert
    OIP106 (TRAK1 Human)
    Use
    Tags
    meGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    188664-plasmid-map-sequence-id-369503

    Enlarge

  15. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51084
    Purpose
    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51084-plasmid-map-sequence-id-172966

    Enlarge

  16. Aiolos AA130-189 in Pea

    Plasmid
    #74459
    Purpose
    Monitor post-translational degradation of Aiolos AA130-189 via EGFP:mCherry ratio (Lentiviral SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry)
    Depositor
    Benjamin Ebert
    Insert
    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    74459-plasmid-map-sequence-id-140419

    Enlarge

  17. pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid
    #115344
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter
    Depositor
    Steven Jacobsen
    Insert
    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    115344-plasmid-map-sequence-id-222905

    Enlarge

  18. pFLAG-CMV-hErk1(K71R)

    Plasmid
    #49329
    Purpose
    Expresses kinase deficient FLAG-tagged human Erk1
    Depositor
    Melanie Cobb
    Insert
    Erk1 (MAPK3 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K71R
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. GOSR2-eGFP

    Plasmid
    #209886
    Purpose
    Expresses human GOSR2 (GS27) fused to eGFP in mammalian cells
    Depositor
    Geert van den Bogaart
    Insert
    GOSR2 (GOSR2 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209886-plasmid-map-sequence-id-413667

    Enlarge

  20. pHD-DsRed-attP-w+

    Plasmid
    #80898
    Purpose
    Vector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    GMR-white
    Use
    High copy, amp resistance
    Tags
    Expression
    Mutation
    Promoter
    3xP3 and GMR::hsp70
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    80898-plasmid-map-sequence-id-172486

    Enlarge

  22. pSG5-HA-ERR3 E275A,R316A

    Plasmid
    #107768
    Purpose
    Expresses a modified receptor with inactivating mutations in key ligand binding residues
    Depositor
    Rebecca Riggins
    Insert
    Estrogen-related receptor gamma (Esrrg Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    E275A,R316A double mutant
    Promoter
    SV40
    Available since
    May 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107768-plasmid-map-sequence-id-209653

    Enlarge

  24. pMSCV_puro_41584

    Plasmid
    #111630
    Purpose
    Complementation of Dicer_KO mESCs with wild type human DICER isoform
    Depositor
    Constance Ciaudo
    Insert
    FLAG-hsDicer (DICER1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    111630-plasmid-map-sequence-id-217596

    Enlarge

  27. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid
    #221158
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mGFP
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    221158-plasmid-map-sequence-id-450962

    Enlarge

  28. CUP1-∆ssCPY*-mScarletI in pRS315

    Plasmid
    #221159
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221159-plasmid-map-sequence-id-450960

    Enlarge

  29. pET50_MDA5∆2CARD

    Plasmid
    #216804
    Purpose
    Expresses melanoma differentiation-associated protein 5 lacking its 2 CARD domains
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ∆1-287
    Promoter
    Available since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    216804-plasmid-map-sequence-id-428835

    Enlarge

  30. pDONR221-TMPRSS11D

    Plasmid
    #158447
    Purpose
    Gateway Cloning compatible entry vector for the human TMPRSS11D gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    TMPRSS11D (TMPRSS11D Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158447-plasmid-map-sequence-id-308252

    Enlarge

  31. GLI2 his

    Plasmid
    #87097
    Purpose
    expresses GLI2 in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    GLI2 (GLI2 Human)
    Use
    Tags
    6x His, T7 tag, and Xpress tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    87097-plasmid-map-sequence-id-201477

    Enlarge

  33. pT7-V5-SBP-C1-HsRRP6

    Plasmid
    #64916
    Purpose
    expresses V5- and SBP-tagged RRP6 in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    RRP6 (EXOSC10 Human)
    Use
    Tags
    V5 and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    64916-plasmid-map-sequence-id-109664

    Enlarge

  34. pGEX6P1-N-Strep2

    Plasmid
    #119758
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.
    Depositor
    Martin Reijns, Andrew Jackson
    Type
    Empty backbone
    Use
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119758-plasmid-map-sequence-id-233666

    Enlarge

  36. pTRIPZ (M)-HT-Cbx4

    Plasmid
    #82513
    Purpose
    Lentivirus vector. Expresses Halotag-Cbx4 fusion proteins in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 4 (Cbx4 Mouse)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82513-plasmid-map-sequence-id-164664

    Enlarge

  37. JMJD3

    Plasmid
    #39069
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JMJD3 (KDM6B Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Promoter
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161817-plasmid-map-sequence-id-320651

    Enlarge

  40. pQE80L-IL2 C125S

    Plasmid
    #104348
    Purpose
    Expresses human IL-2 (C125S)
    Depositor
    Alexander McLellan
    Insert
    Human IL-2 (C125S) (IL2 Human)
    Use
    Tags
    MRGSHHHHHGS-tag
    Expression
    Bacterial
    Mutation
    C125S mutation (TGT -> TCC)
    Promoter
    T5
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104348-plasmid-map-sequence-id-201216

    Enlarge

  41. HaloTag-G protein beta-1

    Plasmid
    #67016
    Purpose
    G protein beta-1 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein beta-1 (GNB1 Human)
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67016-plasmid-map-sequence-id-115474

    Enlarge

  42. pCAG/HA-hBIG1

    Plasmid
    #226253
    Purpose
    Expresses N-terminally HA-tagged hBIG1 in mammalian cells
    Depositor
    Hye-Won Shin
    Insert
    BIG1 (ARFGEF1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226253-plasmid-map-sequence-id-454236

    Enlarge

  43. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid
    #89993
    Purpose
    Donor template for generation of SOX9-ntdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    89993-plasmid-map-sequence-id-180698

    Enlarge

  44. AK2

    Plasmid
    #38829
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AK2 (AK2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  45. Human-Dysferlin-v1

    Plasmid
    #60217
    Purpose
    Human dysferlin exon 1 variant (accession #DQ267935) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60217-plasmid-map-sequence-id-96331

    Enlarge

  46. pTXB1-sfGFP

    Plasmid
    #51562
    Purpose
    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    superfolder GFP
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51562-plasmid-map-sequence-id-78800

    Enlarge

  47. pET28-6His-TEV-Nsp14

    Plasmid
    #154954
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154954-plasmid-map-sequence-id-307815

    Enlarge

  48. FLAG-hSRF (#482)

    Plasmid
    #78343
    Purpose
    Overexpression of human SRF ORF with a N-terminal FLAG tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    SRF (SRF Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  49. AK1

    Plasmid
    #39134
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AK1 (AK1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  50. AK5

    Plasmid
    #39136
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AK5 (AK5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 5151 - 5200 of 8863 results