We narrowed to 4,333 results for: 1-Oct;
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Plasmid#108844PurposeInsert protein fragment into MluI/BspEI to fuse to GID1/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only
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pCin4-3xFLAG-MKL1 L272A
Plasmid#19850DepositorInsertMKL1 L272A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Leucine 272 to AlaninePromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 S449A
Plasmid#19842DepositorInsertMKL1 S449A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Serine 449 to AlaninePromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-RUNX1-WT
Plasmid#238536PurposeUsed as a positive control for overexpression of human RUNX1 in mammalian cells.DepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterMSCV and PGKAvailable sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ 3xFLAG-FEM1B C186S
Plasmid#230995PurposeTransient overexpression of mouse FEM1B in mammalian cells. Mutation abolishes substrate binding.DepositorInsertFEM1B (Fem1b Mouse)
UseTags3xFLAGExpressionMammalianMutationmutated Cys186 to SerPromoterAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ 3xFLAG-FEM1B L597A
Plasmid#230994PurposeTransient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2.DepositorInsertFEM1B (Fem1b Mouse)
UseTags3xFLAGExpressionMammalianMutationmutated Leu597 to AlaPromoterAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Drosophila Wdr20-V5
Plasmid#217622PurposeMammalian expression of V5 tagged Drosophila Wdr20DepositorInsertWDR20
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-Q2L7-T2176C-UGI-Puro
Plasmid#209648PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-Q2L7-T2176C
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-FZY2-S100N-UGI-Puro
Plasmid#209644PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-FZY2-S100N
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-FZY2-S100C-UGI-Puro
Plasmid#209643PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-FZY2-S100C
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 CortW22A mCherry
Plasmid#187268PurposeLenticiral expression of mCherry-Cortactin W22ADepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmcherryExpressionMutationW22APromoter5' LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA
Plasmid#173211PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
UseCRISPR; Prime editing (pe3)TagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA
Plasmid#173212PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-bPAC(R278A)-Myc-eYFP
Plasmid#165488PurposeXenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activityDepositorInsertbPAC(R278A)-Myc-eYFP
UseCdna expression, xenopus oocyteTagsEYFP and MycExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES-EGFP-TPD53
Plasmid#172461PurposeExpression of untagged Tumor Protein D52 like 1 (TPD53/TPD52L1) and GFPDepositorInsertTPD53 (TPD52L1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
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NH2end ELL2
Plasmid#127252PurposeExpresses the N terminal segment of human ELL2 in mammalian cellsDepositorInsertELL2 (1-287) (ELL2 Human)
UseOtherTagsHis6XExpressionMammalianMutationcontains only amino acids 1-289PromoterpEF1aAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-I894A(pL84)
Plasmid#124404PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.DepositorInsertGal4-TET1-878-911(I894A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationI894APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-T898A(pL84)
Plasmid#124403PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.DepositorInsertGal4-TET1-878-911(T898A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationT898APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L900A(pL84)
Plasmid#124402PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.DepositorInsertGal4-TET1-878-911(L900A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationL900APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only