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hARL15_32-197

Plasmid

#209799

Purpose

Bacterial expression of human ARL15 GTPase domain

Depositor

Kalle Gehring

Article

Mahbub et al Elife. 2023 Jul 14;12:e86129. doi: 10.75

Insert

ARL15 (ARL15 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 32-197 only

Promoter

T7

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_I3969A/F3982A

Plasmid

#187129

Purpose

mammalian cell expression of 3xFLAG-HUWE1 I3969A/F3982A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

I3969A/F3982A

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_102-330-V5His6_D

Plasmid

#146129

Purpose

Insect Expression of DmEDC3_102-330

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3_102-330 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-2xStrep-ORF66 1-200

Plasmid

#130954

Purpose

Expresses KSHV ORF66 a.a. 1-200 with an N-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Tags

2xStrep

Expression

Mammalian

Mutation

ORF66 a.a. 1-200

Promoter

CMV

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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mei-S332 5.6Kb genomic DNA in Casper4

Plasmid

#112984

Purpose

P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

Insert

mei-S332 (Sgo) (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 PUM FL

Plasmid

#112997

Purpose

For protein expression and purification of Drosophila pumilio (amino acids 566-1090)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

pumilio (pum Fly)

Expression

Bacterial

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA GNSTM-3-RVG-10-Lamp2b-HA

Plasmid

#71294

Purpose

Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

Depositor

Joshua Leonard

Article

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Tags

GNSTM glycosylation motif, HA, Lamp2 signal pepti…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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Basigin-isoform2-CD4d3+4-bio

Plasmid

#73129

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Basigin-isoform2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Basigin isoform 2 (BSG Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_NRF2

Plasmid

#159129

Purpose

expresses NRF2 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

NRF2 (NFE2L2 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLenSyn2-EGFP-OMP25C

Plasmid

#71427

Purpose

Lentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.

Depositor

Henri Huttunen

Article

Kysenius et al PLoS One. 2014 Sep 5;9(9):e107129. doi:

Insert

OMP25 (172-‐206) (Synj2bp Rat)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 172-206 only

Promoter

synapsin

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
TALED_Right-mouse-ATP6-1397C-AD(V28R)

Plasmid

#212955

Purpose

Expresses TALED_Right-mouse-ATP6-1397C-AD(V28R) in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

Right-mouse-ATP6-1397C-AD(V28R) (ATP6 Synthetic)

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgACSL4 clone2

Plasmid

#162129

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A

Plasmid

#212949

Purpose

Expresses TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A (mt-Rnr1 Synthetic)

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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PUM1_PUM_pGEX

Plasmid

#135129

Purpose

Recombinant expression of PUM1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

PUM1 (PUM1 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-FLIP pr4

Plasmid

#19129

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

FLIP promoter (CFLAR Human)

Use

Luciferase

Expression

Mammalian

Mutation

The sequence corresponds to positions -503 to +10…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pGL4-Cluster Promoter Delta 1.1

Plasmid

#100129

Purpose

It contains ~0.8 Kb of the miR-183-96-182 cluster promoter. Is the minimal region that contains the maximal promoter activity

Depositor

Eva Hernando

Article

Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

Insert

miR-183-96-82 Cluster promoter 0.8 Kb Fragment (MIR182 Human)

Use

Luciferase

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-R59P

Plasmid

#248129

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with R59P mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-R59P (SNAP25 Human)

Expression

Mammalian

Mutation

R59P

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHCFnc/V5

Plasmid

#247003

Purpose

Expresses non cleavable HCF-1 with carboxy terminal V5 tag under control of the CMV promoter

Depositor

Thomas Kristie

Article

Vogel et al Proc Natl Acad Sci U S A. 2006 May 2;103

Insert

HCF-1 (HCFC1 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

Glu to Ala change at amino acid positions 1019,10…

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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NPM1c-TurboID-MSCV-IRES-GFP

Plasmid

#240129

Purpose

Expresses mutant NPM1c-TurboID fusion in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

NPM1c (NPM1 Human)

Use

Retroviral

Tags

IRES-GFP and TurboID

Expression

Mammalian

Mutation

C-terminal frameshift mutation as seen in Acute M…

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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EW378 (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-GFP

Plasmid

#236134

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with four ZNF35/ZNF250 bindi…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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