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C078

Plasmid

#174347

Purpose

Template for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

ePAB

Use

In vitro transcription template

Tags

FLAG

Mutation

13 silent mutations: G243A, A273G, G339A, C345G, …

Availability

Academic Institutions and Nonprofits only

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C233

Plasmid

#174349

Purpose

Template for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (M161A) (pabpc1.S Frog)

Use

In vitro transcription template

Tags

FLAG

Mutation

M161A

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mito-TagRFP-T

Plasmid

#174543

Purpose

labels mitochondria with TagRFP-T

Depositor

Minna Roh-Johnson

Article

Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

Insert

MTS-tagRFP-T

Use

Lentiviral

Tags

tagRFP-T

Expression

Mammalian

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 3xHA-KillerRed-OMP25

Plasmid

#174544

Purpose

localizes KillerRed to mitochondria

Depositor

Minna Roh-Johnson

Article

Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

Insert

3xHA-KillerRed-OMP25

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6-H2B-mCherry

Plasmid

#89766

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged histone H2B

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

Histone H2B (H2BC11 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV4-p105

Plasmid

#23288

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

p105 (NFKB1 Human)

Expression

Mammalian

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-RelA

Plasmid

#23255

Depositor

Warner Greene

Article

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Insert

p65 (RELA Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

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3X ERE TATA luc

Plasmid

#11354

Purpose

Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

Depositor

Donald McDonnell

Article

Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

Insert

ERE

Use

Luciferase

Expression

Bacterial

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ1-promoterless-LacZ

Plasmid

#191617

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pKG2440_pGEEC536_pShip-UbC-mRuby2-P2A-PuroR-bGH

Plasmid

#239732

Purpose

Plasmid expressing mRuby2 and PuroR with the UbC promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-DFCP1

Plasmid

#86746

Purpose

N-terminal mCherry-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human DFCP1 (ZFYVE1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pKG1988_pPV1-UbC

Plasmid

#239689

Purpose

BsaI Golden Gate assemby plasmid encoding the UbC promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

UbC promoter

Use

Synthetic Biology

Mutation

Removal of BsaI cut sites

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-epsilon-14-3-3

Plasmid

#250063

Purpose

Bacterial expression of epsilon 14-3-3

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Epsilon (YWHAE Human)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta K85A pcDNA3

Plasmid

#14755

Depositor

Jim Woodgett

Article

Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

Insert

GSK3 beta K85A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

K85A. Kinase dead mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pGW1-Myc-Sarm1

Plasmid

#50707

Purpose

expression of Sarm1 in mammalian cells

Depositor

Yi-Ping Hsueh

Article

Chen et al J Cell Biol. 2011 May 16;193(4):769-84.

Insert

Sarm1 (Sarm1 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only
CCR5-donor-MCS

Plasmid

#192269

Purpose

Donor template for CRISPR Genome Editing

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

MCS within donor template

Use

CRISPR

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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3922-48

Plasmid

#176497

Purpose

pTRE-EGFP-T2A-TFEB(wt)-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

EGFP-T2A-TFEB(wild-type; full-length; human)

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV4-p100

Plasmid

#23287

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

NFkB p100 subunit (NFKB2 Human)

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pGFP-AUG-IRES-mCherry

Plasmid

#222109

Purpose

Start codon reporter (WT AUG)

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV4-p52

Plasmid

#23289

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

p52 (NFKB2 Human)

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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