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Plasmid#218891Purposegolden gate cloningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only
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pLV-W-ccdB-Z
Plasmid#218890Purposegolden gate cloningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5(PrD)
Plasmid#166167PurposeGateway entry vector for N. furzeri DDX5-PrD(535-634) mutantDepositorArticleInsertDDX5-PrD(535-634)
UseGateway entry vectorTagsExpressionMutationPrD only 1606-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5ΔPrD
Plasmid#166164PurposeGateway entry vector for N. furzeri DDX5ΔPrD(1-535) mutantDepositorArticleInsertDDX5ΔPrD(1-535)
UseGateway entry vectorTagsExpressionMutationdeletion of 1606-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5ΔIDR
Plasmid#166165PurposeGateway entry vector for N. furzeri DDX5ΔIDR(1-483) mutantDepositorArticleInsertDDX5ΔIDR(1-483)
UseGateway entry vectorTagsExpressionMutationdeletion of 1450-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5(IDR)
Plasmid#166166PurposeGateway entry vector for N. furzeri DDX5-IDR(483-634) mutantDepositorArticleInsertDDX5-IDR(483-634)
UseGateway entry vectorTagsExpressionMutationIDR only 1450-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
ipGL2Cas
Plasmid#210555PurposeBinary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by GL2 promoter.DepositorInsertGL2p::XVE::UBQ10t::LexA::SpCas9::PEA3AT::VenusSSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
ipYUC2Cas-LT1
Plasmid#210559PurposeBinary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter.DepositorInsertpMpEF1::XVE::UBQ10t::LexA::SpCas9::PEA3AT::pU6::AAVS1gRNA::pMpEF1::LT1::Hygromycin
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1Cas-LT1
Plasmid#210556PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1pDepositorInsertpMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGC-LexACasPea
Plasmid#210539PurposeCloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module CDepositorInsertLexA::SpCas9::PEA3AT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGA-pCLV3
Plasmid#210528PurposeCloning A. thaliana CLV3 promoter as GreenGate module ADepositorInsertpCLV3 (CLV3 Mustard Weed)
UseSynthetic Biology; Golden gate compatible cloning…TagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIT348
Plasmid#204515PurposeBacterial expression of CEC-5 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertC-terminal fragment of cec-5 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MLKS2ec
Plasmid#130855PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2exp
Plasmid#130856PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseTagseGFP-FLAG-HAExpressionPlantMutationPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHIT353
Plasmid#204506PurposeIn vitro transcription of RNA probes for in situ hybridization of CeRep55 lncRNAs in nematode (after linearization with NotI and BglI)DepositorInsertCeRep55 tandem repeats from Y73B3A (C. elegans)
UseOtherTagsExpressionMutationPromoterdouble-T7Available sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b_TypeIC_acrIF2_invivo
Plasmid#196402Purposeexpresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assayDepositorInsertanti-CRISPR IF2 protein (orf30 P. aeruginosa)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT334
Plasmid#204516PurposeBacterial expression of COH-3 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertC-terminal fragment of coh-3 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT203
Plasmid#204512PurposeBacterial expression of C04F12.1 with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertC04F12.1 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT517
Plasmid#204518PurposeBacterial expression of CEC-4 with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertcec-4 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT521
Plasmid#204520PurposeBacterial expression of CEC-5 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertN-terminal fragment of cec-5 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only