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Plasmid#138225PurposeExpresses the split mCherry reporter interrupted by the Cro-RIR1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only
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pFP.R356
Plasmid#138226PurposeExpresses the split mCherry reporter interrupted by the Cro-RPB2 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R357
Plasmid#138227PurposeExpresses the split mCherry reporter interrupted by the Cro-Top2 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R358
Plasmid#138228PurposeExpresses the split mCherry reporter interrupted by the CIV-RIR1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R359
Plasmid#138229PurposeExpresses the split mCherry reporter interrupted by the CP-Thy1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R361
Plasmid#138231PurposeExpresses the split mCherry reporter interrupted by the BsuP-RIR1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLinkC-AI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMV
Plasmid#137115PurposeProtease Switch Clone ID: 1-A9DepositorInsertAI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLinkC-AI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV
Plasmid#137116PurposeProtease Switch Clone ID: 1-C9DepositorInsertAI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LOC5570372 NPFF receptor
Plasmid#115378PurposeExpresses Ae. aegypti receptor in mammalian cellsDepositorInsertLOC5570372 NPFF receptor (LOC5570372 Aedes aegypti)
UseTagsExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSO9
Plasmid#106366PurposeTo insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the intestine.DepositorInsertttTi4348_ SEC _pvit-6_OspF_3'unc54
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FFSARG-ddFLN4-HIS-ybbr
Plasmid#101240Purposeminimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFFSARG peptide, ddFLN4 fingerprint
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFPJ1
Plasmid#83561PurposeExpress yEGFP-ERG9 ER signal peptide under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER signal peptide
UseTagsExpressionYeastMutationPromoterTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA186
Plasmid#74250PurposeIntermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).DepositorInsertInducible peptide of the dPSTR
UseTagsExpressionBacterial and YeastMutationPromoterpGPD1Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA171
Plasmid#74251PurposeIntermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
UseTagsVenusExpressionBacterial and YeastMutationPromoterpSTL1Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1 R123A
Plasmid#66721PurposeConstitutive expression of FPR1 R123A in mammalian cells.DepositorInsertFPR1 (haplotype 3) (FPR1 Human)
UseTagsExpressionMammalianMutationR123APromoterSR-alphaAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1 D71A
Plasmid#66720PurposeConstitutive expression of FPR1 D71A in mammalian cells.DepositorInsertFPR1 (haplotype 3) (FPR1 Human)
UseTagsExpressionMammalianMutationD71APromoterSR-alphaAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1 N297A
Plasmid#66723PurposeConstitutive expression of FPR1 N297A in mammalian cells.DepositorInsertFPR1 (haplotype 3) (FPR1 Human)
UseTagsExpressionMammalianMutationN297APromoterSR-alphaAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1 Q258A
Plasmid#66724PurposeConstitutive expression of FPR1 Q258A in mammalian cells.DepositorInsertFPR1 (haplotype 3) (FPR1 Human)
UseTagsExpressionMammalianMutationQ258APromoterSR-alphaAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAKW.114A-PKA
Plasmid#154051PurposeExpresses PKA in E. coliDepositorInsertProtein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
UseTagsExpressionBacterialMutationPromoterpCinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Azi
Plasmid#105829PurposePlasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cellsDepositorInsertsHumanized EAziRS
4 copies of the U6-BstYam tRNA cassette
UseTagsExpressionMammalianMutation(Y37L, D182S, F183M, L186A, D265R)TyrRSPromoterPGK and U6Available sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only