165,947 results

1. pTR-374 tNGFR-P2A-B2M

   Plasmid

   #186068

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   B2M (B2M Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbS8k-RFP_R43W_E93G

   Plasmid

   #109406

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   R43W and E93G repA mutations to increase copy num…

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRetroX-TRE3G_NT-GSDMD_I105N_

   Plasmid

   #201174

   Purpose

   Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D N-terminal domain (Gsdmd Mouse)
   

   Use

   Retroviral

   Mutation

   aa1-276 only, I105N

   Promoter

   TRE3G

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. P2RY8-Tango

   Plasmid

   #66477

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   P2RY8 (P2RY8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pEntr1a-mcherry-FKBP-TIAM1(DHPH)

   Plasmid

   #176135

   Purpose

   Heterodimerization with FRB (rapamycin), Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Entry vector

   Mutation

   See Depositor Comments Below

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIBN-LacI

   Plasmid

   #103814

   Purpose

   BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-LacI (CIB1, lacI E. coli, Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   LacI: C19T (silent, L7L), T264C (silent, A88A), C…

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRESpuro3+mRFP-Utr_CH

   Plasmid

   #64358

   Purpose

   bicistronic expression of Utrophin-mRFP and Puro resistance

   Depositor

   Matthieu Piel

   Insert

   Utrophin-mRFP (UTRN Human)
   

   Tags

   IRES-Puro and mRFP

   Expression

   Mammalian

   Mutation

   Q236R; aa1-261 Utrophin present

   Promoter

   CMV

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_SLC35G2

   Plasmid

   #132144

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC35G2 (TMEM22 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR221_SLC35G6

   Plasmid

   #132096

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC35G6 (SLC35G6 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 2, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. R777-E180 Hs.PIK3R5-nostop

    Plasmid

    #70464

    Purpose

    Gateway ORF clone of human PIK3R5 [NM_014308.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PIK3R5 (PIK3R5 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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