Addgene

We narrowed to 14,719 results for: nans

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Dendra-Rab11a

Plasmid

#184038

Purpose

Expresses red to green photoconvertible Rab11a

Depositor

Heidi Hehnly

Article

Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

Insert

Rab11 (RAB11A Human)

Use

Tags

Dendra

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128345

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrnb4 (Chrnb4 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pVSV-G

Plasmid

#138479

Purpose

Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

VSV-G envelop protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#183951

Purpose

Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

APOBEC1 (Apobec1 Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/38.1R]

Plasmid

#114492

Purpose

Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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SUPERBLADE5

Plasmid

#134913

Purpose

Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

Plasmid

#164822

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

miniCMV and pGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_CRATES

Plasmid

#176251

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1

Plasmid

#176250

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozym

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR and Synthetic Biology; Expression in micro…

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EFNB2-RBD-Fc (WT)

Plasmid

#200978

Purpose

Mammalian expression plasmid for soluble EFNB2 (wild type) fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Use

Tags

IgG1 Fc

Expression

Mammalian

Mutation

Soluble Receptor-Binding Domain

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pIZ-EGFP-MYO10-HMM

Plasmid

#87257

Purpose

Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Use

Tags

Expression

Insect

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

OpIE2

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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EF.STAT3DN.Ubc.GFP

Plasmid

#24984

Purpose

Depositor

Linzhao Cheng

Article

Hillion et al Cancer Research 68, 10121, December 2008

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Y705F--dominant negative

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/38]

Plasmid

#182095

Purpose

Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GFP

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EphB2

Plasmid

#200982

Purpose

Mammalian expression plasmid for myc-tagged EphB2

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB2 (EPHB2 Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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K785R Smarca4-sfGFP

Plasmid

#107057

Purpose

Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

Depositor

Jerry Crabtree, Courtney Hodges

Article

Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)

Use

Lentiviral

Tags

Super-folder GFP (sfGFP)

Expression

Mammalian

Mutation

K785R

Promoter

CAG

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pDiCre-mCherry

Plasmid

#164572

Purpose

Integration of a DiCre cassette in PbGFP parasite genome

Depositor

Olivier Silvie

Article

Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. d

Inserts

FKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Bidirectional PbEF1alpha promoter (PBANKA_1133300…

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183

Plasmid

#164827

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

miniCMV and pGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Medium_SynNotch_RHL144

Plasmid

#164828

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Medium Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Medium Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

miniCMV and pGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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(383) pcDNA3.1-HA-nBC2-OGA(544-706)

Plasmid

#168100

Purpose

HA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

HA-nBC2-OGA(544-706)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

OGA truncated to contain residues 544-706

Promoter

T7/CMV

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGLOW39

Plasmid

#173068

Purpose

mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

mScarlet-I-C1^SEC^3xMyc

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mScarlet

Expression

Worm

Mutation

Promoter

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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