2,294 results

1. pcDNA-HisFlag-rUSP19ER deltaN3

   Plasmid

   #155243

   Purpose

   for mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (delta1-1631) (ER isoform) (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA-HisFlag-rUSP19ER deltaN2

   Plasmid

   #155242

   Purpose

   for mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (delta1-1088) (ER isoform) (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA-HisFlag-rUSP19ER deltaN1

   Plasmid

   #155241

   Purpose

   for mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (delta1-536) (ER isoform) (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-HisFlag-rUSP19CYT

   Plasmid

   #155238

   Purpose

   for mammalian overexpression of the cytoplasmic isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (Cytoplasmic isoform) (Usp19 Rat, Mouse)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCIS-rPALcc

   Plasmid

   #145804

   Purpose

   expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells

   Depositor

   Betty Eipper, Richard Mains

   Article

   Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9

   Insert

   rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
   

   Expression

   Mammalian

   Mutation

   changed serine 767 to alanine to prevent glycosyl…

   Promoter

   CMV

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mTurquoise2-RnBarr2

   Plasmid

   #137798

   Purpose

   Visualization of beta-arrestin2

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat beta-arrestin2 (Arrb2 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mTurquoise2-RnBarr1

   Plasmid

   #137795

   Purpose

   Visualization of beta-arrestin2

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat beta-arrestin 1 (Arrb1 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RnAT1aR DRY->AAY-p2A-mCherry

   Plasmid

   #137784

   Purpose

   Visualization of the At1aR DRY mutant

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat At1a receptor; DRY mutant (Agtr1a Rat)
   

   Expression

   Mammalian

   Mutation

   DRY -> AAY

   Promoter

   CMV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RnAT1aR DRY->AAY-mCherry

   Plasmid

   #137783

   Purpose

   Visualization of the At1aR DRY mutant

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat At1a receptor; DRY mutant (Agtr1a Rat)
   

   Expression

   Mammalian

   Mutation

   DRY -> AAY

   Promoter

   CMV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MTK3b_015

    Plasmid

    #123796

    Purpose

    Encodes rat Connexin 26 as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Cx 26 (rat)
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MTK3b_017

    Plasmid

    #123798

    Purpose

    Encodes rat Connexin 43 as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Cx43 (rat)
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MBP-rPKL S12TAG

    Plasmid

    #107155

    Purpose

    MBP-rPKL construct for production and purification of rat PKL pS12

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Serine 12 to TAG for phosphoserine insertion

    Promoter

    PBAD

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MBP-rPKL S113TAG

    Plasmid

    #107157

    Purpose

    MBP-rPKL S113TAG for production and purification of rat PKL pS113

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Serine 113 changed to TAG for phosphoserine inser…

    Promoter

    PBAD

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. prNFL-P22S-EGFP

    Plasmid

    #132594

    Purpose

    Expresses hEGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P22S mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    NEFL (Nefl Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    64C>A, g5C>G, Pro22Ser

    Promoter

    CMV

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPRIME-CMV-GFP-shNts2

    Plasmid

    #132714

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Nts

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(2) (Nts Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPRIME-CMV-GFP-shNts1

    Plasmid

    #132713

    Purpose

    Encodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(1) (Nts Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPRIME-CMV-GFP-shCrh2

    Plasmid

    #132710

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shCrh(2) (Crh Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2+ rat MYO1D

    Plasmid

    #118419

    Purpose

    For mammalian expression of rat myosin 1D

    Depositor

    Michael Tsang

    Article

    Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

    Insert

    rat myosin 1D (Myo1d Rat)
    

    Expression

    Mammalian

    Available Since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHybr_r2a>Fab-srt-his

    Plasmid

    #124810

    Purpose

    HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.

    Depositor

    Martijn Verdoes, Ferenc Scheeren

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    rat IgG2a Hinge-G4S-Sortag-Histag
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial

    Promoter

    pUC

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FH95pUP95S73AGW (F2)

    Plasmid

    #74039

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-S73A-EGFP fusion

    Depositor

    Bernardo Sabatini, Robert Malenka, Weifeng Xu

    Article

    Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Mutation

    S73A

    Promoter

    H1

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only