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Showing: 2081 - 2100 of 40900 results

  2. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid
    #128386
    Purpose
    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption
    Depositor
    Craig Duvall
    Article
    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss
    Inserts
    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Synthetic, Human)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    C-terminal luc2 fragment and N-terminal luc2 frag…
    Expression
    Mammalian
    Mutation
    N terminal carbohydrate recognition domain fused …
    Promoter
    EF1A and IRES
    Available Since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    128386-plasmid-map-sequence-id-263327

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  3. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid
    #177334
    Purpose
    bacterial expression of active SARS-CoV-2 3C-like proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177334-plasmid-map-sequence-id-348602

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  4. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid
    #224697
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224697-plasmid-map-sequence-id-444276

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  5. Anti-Kv4.3 K+ channel [K75/41-rat IgG2a] - Chimeric

    Plasmid
    #227058
    Purpose
    Mammalian expression plasmid of anti-Kv4.3 K+ channel (Rattus norvegicus) rat IgG2a R-mAb. Derived from hybridoma K75/41.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Kv4.3 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody. (Kcnd3 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    227058-plasmid-map-sequence-id-450506

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  6. pFFLuc-IVT

    Plasmid
    #205147
    Purpose
    IVT template of FireFly luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    FireFly luciferase
    Use
    Luciferase; In-vitro transcription
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205147-plasmid-map-sequence-id-495367

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  7. pRLuc-IVT

    Plasmid
    #205148
    Purpose
    IVT template of Renilla luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    Renilla Luciferase
    Use
    Luciferase; In-vitro transcription
    Promoter
    T7
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205148-plasmid-map-sequence-id-404883

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  8. RL-Mos

    Plasmid
    #120519
    Purpose
    Renilla luciferase reporter
    Depositor
    Petr Svoboda
    Insert
    Renilla luciferase (Mos Synthetic, Mouse)
    Use
    Luciferase
    Available Since
    Jan. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    120519-plasmid-map-sequence-id-233809

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  9. pcDNA3.1-N-Luc

    Plasmid
    #185357
    Purpose
    For mammalian expression of N-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 2-416).
    Depositor
    Ayaz Najafov
    Insert
    Firefly Luciferase
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    185357-plasmid-map-sequence-id-363972

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  10. pcDNA3.1-C-Luc

    Plasmid
    #185358
    Purpose
    For mammalian expression of C-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 398-550).
    Depositor
    Ayaz Najafov
    Insert
    Firefly Luciferase
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    185358-plasmid-map-sequence-id-376433

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  11. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid
    #177335
    Purpose
    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177335-plasmid-map-sequence-id-348601

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  12. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid
    #224698
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224698-plasmid-map-sequence-id-444264

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  13. pUC-ODN2

    Plasmid
    #208643
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. Further remove BsaI and BsmBI site in pUC-ODN (Addgene #208642).
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208643-plasmid-map-sequence-id-418356

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  14. pcDNA3.1-3'3xFlag

    Plasmid
    #208616
    Purpose
    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208616-plasmid-map-sequence-id-418312

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  16. pcDNA3.1-StrepII

    Plasmid
    #208617
    Purpose
    Empty vector for mammalian expression, insert a Strep II tag (with ATG) using XbaI and XhoI site of pcDNA3.1 (-), both site retained
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Strep II
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208617-plasmid-map-sequence-id-418313

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  17. pCLX-KRT7-GFP

    Plasmid
    #216853
    Purpose
    This plasmid is for the production of lentiviral vectors that can be used to identify and live sort pancreatic progenitor expresssing the Keratin7 gene.
    Depositor
    Patrick Salmon
    Insert
    GFP
    Use
    Lentiviral
    Promoter
    Keratin7
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216853-plasmid-map-sequence-id-431654

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  18. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    6xhis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208635-plasmid-map-sequence-id-433640

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  19. pcDNA3.1-3'TwStrep

    Plasmid
    #208618
    Purpose
    Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Twin Strep
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208618-plasmid-map-sequence-id-418314

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  20. pUC-ODN

    Plasmid
    #208642
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208642-plasmid-map-sequence-id-418350

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Showing: 2081 - 2100 of 40900 results