We narrowed to 4,866 results for: IRES

1. pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

   Plasmid

   #167832

   Purpose

   Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   HRAS(N17) (HRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   S17N

   Promoter

   Ubc, TRE

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

   Plasmid

   #216695

   Purpose

   Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Inserts

   FIREmate-KDEL
   ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
   

   Use

   Tags

   FIREtag, Streptavidin Binding Protein (SBP), and …

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S

   Plasmid

   #62460

   Purpose

   expresses C151S and C288S double mutant human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Use

   Tags

   His-Halo-Tev

   Expression

   Mammalian

   Mutation

   Changed Cysteines 151 and 288 of Keap1 to respect…

   Promoter

   CMV

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

   Plasmid

   #229839

   Purpose

   A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

   Plasmid

   #89587

   Purpose

   For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   nuclear localization signal (nls) turboRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL3.7 K122 -ires-GFP-Osteocalcin-promoter-H2B mCherry reporter

   Plasmid

   #68714

   Depositor

   Yutaka Hata

   Insert

   H2B mCherry and GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Chx10 (164bp crit reg+SV40 bas prom)-GFP-IRES-AP

   Plasmid

   #18808

   Depositor

   Connie Cepko

   Article

   Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

   Insert

   Chx10 (164bp crit reg+SV40 bas prom)-GFP-IRES-AP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

8. NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

   Plasmid

   #89994

   Purpose

   Donor template for generation of NGN3-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry

   Plasmid

   #122959

   Purpose

   Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expression

   Depositor

   Pierre Vanderhaeghen

   Article

   Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

   Insert

   NOTCH2NL-delta C terminus (NOTCH2NLB Human)
   

   Use

   Lentiviral

   Tags

   6xmyc tag

   Expression

   Mutation

   deletion of C terminus from full length NOTCH2NLB

   Promoter

   CAG promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP

    Plasmid

    #122956

    Purpose

    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Expression

    Mutation

    deletion of C terminus from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. XZ066-(PB)EF1a-LIDARST-CCR6V2-IRESv0-T2A-PuroR-P2A-BFP

    Plasmid

    #233418

    Purpose

    PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available since

    June 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. XZ079 (PB)EF1a-CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

    Plasmid

    #233435

    Purpose

    PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. LSM042 (PB)EF1a-AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

    Plasmid

    #233426

    Purpose

    PiggyBac donor plasmid AVPR2-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9

    Plasmid

    #231910

    Purpose

    For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

    Plasmid

    #229845

    Purpose

    A CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Lamin nanobody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD)

    Plasmid

    #231235

    Purpose

    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TAD deleted (delta1-143), DBD deleted (delta340-4…

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-miRFP680-NES-p2A-ezrin-T567A-mRuby3-IRES-Neo

    Plasmid

    #222637

    Purpose

    Ezrin activation reporter, T567 phosphorylation deficient

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    miRFP680-NES-p2A-ezrin-T567A-mRuby3 (EZR Human)
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    T567A

    Promoter

    EF1a

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

    Plasmid

    #218636

    Purpose

    Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    H781E, Q782R, F785A

    Promoter

    UBC

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

    Plasmid

    #218635

    Purpose

    Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    N1008R, R1009E, E1010R, R1013E

    Promoter

    UBC

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)

    Plasmid

    #190755

    Purpose

    Expression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.

    Depositor

    Dave Piston

    Article

    Foust et al bioRxiv 2021.11.03.467169

    Inserts

    GNB1 (GNB1 Human)
    GNG2 (GNG2 Human)
    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    mCherry2 and mEYFP(Q69K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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