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pGL3-pSMANTIS_STAT1-2_Mutant

Plasmid

#194189

Purpose

Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

mutated STAT1/2 site, Region 653-673nt of 1000nt

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-VRER-P2A-GFP-PGK-Puro

Plasmid

#110862

Purpose

Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRER

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-xF2X-P2A-Puro

Plasmid

#110873

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS-2X(3.7)

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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TRAV29/DV5

Plasmid

#237986

Purpose

Encodes TRAV29/DV5 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV29/DV5 (TRAV29DV5 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV14/DV4

Plasmid

#237972

Purpose

Encodes TRAV14/DV4 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV14/DV4 (TRAV14DV4 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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His-PHD2-181-426

Plasmid

#223551

Purpose

His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-180.

Promoter

trc

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

Plasmid

#194190

Purpose

Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

5 mutated KLF binding sites (positions 449,548,73…

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 899

Plasmid

#194193

Purpose

Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

mutated KLF binding sites (position 899)

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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TRAV38-2/DV8

Plasmid

#237992

Purpose

Encodes TRAV38-2/DV8 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV38-2/DV8 (TRAV38-2DV8 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV36/DV7

Plasmid

#237990

Purpose

Encodes TRAV36/DV7 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV36/DV7 (TRAV36DV7 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV23/DV6

Plasmid

#237980

Purpose

Encodes TRAV23/DV6 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV23/DV6 (TRAV23DV6 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV18

Plasmid

#237975

Purpose

Encodes TRAV18 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV18 (TRAV18, TRBC1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 732

Plasmid

#194195

Purpose

Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 732)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

mutated KLF binding sites (position 732)

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 884

Plasmid

#194196

Purpose

Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 884)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

mutated KLF binding sites (position 884)

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 449

Plasmid

#194197

Purpose

Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 449)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

mutated KLF binding sites (position 449)

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)

Plasmid

#194191

Purpose

Includes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

4 mutated KLF binding sites (positions 449,548,73…

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 449,548,732 (Tri)

Plasmid

#194192

Purpose

Includes the promoter (1kb) of SMTS with 3 mutated KLF binding sites (positions 449,548,732)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

3 mutated KLF binding sites (positions 449,548,73…

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-pSMANTIS_1kb_KLF2mut 548

Plasmid

#194194

Purpose

Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 548)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

mutated KLF binding sites (position 548)

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-10xHis

Plasmid

#194178

Purpose

Expresses MPP8-10xHis tagged

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

Plasmid

#169161

Purpose

To express nsp10-nsp14 fusion protein in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab SARS-CoV-2, Synthetic)

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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