We narrowed to 3,167 results for: tef

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bRA66-deltaMic60

Plasmid

#245524

Purpose

Edited bRA66 containing gRNA to create S. cerevisiae deltaMic60 and S. cerevisiae Mic60(1-317)

Depositor

Luke Chao

Publication

Benning et al Protein Sci. 2025 Jul;34(7):e70207. doi:

Insert

YKR016w (MIC60 Budding Yeast)

Use

CRISPR

Promoter

T7 TAATACGACTCACTATAGG

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pYAMTrGCsgScLEU2

Plasmid

#224869

Purpose

Disruption of S. cerevisiae type LEU2 genes

Depositor

Katsunori Suzuki

Publication

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0617 (pDEST-mEGFP C-term, NatR)

Plasmid

#210484

Purpose

pDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

mEGFP

Expression

Yeast

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK23 pCAS-Tyr-[gRNA: 6=ARS416] (SplitHygR, AmpR)

Plasmid

#179007

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR)

Plasmid

#179006

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK17 pCAS-Tyr-[gRNA: 7=HIS3] (SplitKanR, AmpR)

Plasmid

#179001

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK16 pCAS-Tyr-[gRNA: 6=ARS416] (SplitKanR, AmpR)

Plasmid

#179000

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR)

Plasmid

#178999

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR)

Plasmid

#178998

Purpose

Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK13 pCAS-Tyr-[gRNA: 3=XII-2] (SplitKanR, AmpR)

Plasmid

#178997

Purpose

Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUDE724

Plasmid

#103023

Purpose

expression of a Cpf1 programming crRNA targetting PDR12 (crPDR12-3S)

Depositor

Jean-Marc Daran

Publication

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crPDR12-3.S (PDR12 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCN-LYS21-GFP

Plasmid

#45335

Depositor

Brendan Cormack

Publication

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

yeGFP

Use

C. glabrata expression

Promoter

LYS21

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pAG36-NPL3-3xHA

Plasmid

#262529

Purpose

Expresses NPL3 with C-terminal 3xHA tag under its native promoter

Depositor

Michael Downey

Insert

Npl3 (NPL3 Budding Yeast)

Tags

3xHA

Expression

Yeast

Mutation

Sequence has been codon optimized

Promoter

Native

Available since

Oct. 8, 2026

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-Z3-Nat-ADE2

Plasmid

#232106

Purpose

Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Publication

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

ADE2 gRNA and repair template

Use

CRISPR

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

Z3

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-Z3-Hyg-ADE2

Plasmid

#232107

Purpose

Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Publication

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

ADE2 gRNA and repair template

Use

CRISPR

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

Z3

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgSc-SeLEU2

Plasmid

#224871

Purpose

Disruption of LEU2 genes in the Saccharomyces sense strict group species

Depositor

Katsunori Suzuki

Publication

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence Sc-SeLEU2 (LEU2, DI49_0736 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYea5.1_C99_sfGFP

Plasmid

#207802

Purpose

Yeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4

Depositor

Dieter Langosch

Publication

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-C100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch3_sfGFP

Plasmid

#207864

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4

Depositor

Dieter Langosch

Publication

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch3_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pROS_phleo-Tps1/Gsy2

Plasmid

#196612

Purpose

Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

Depositor

Matthias Heinemann

Publication

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

TPS1, GSY2 (TPS1, GSY2 Budding Yeast)

Expression

Yeast

Promoter

SNR52

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pROS13-Tps2/Gsy1

Plasmid

#196613

Purpose

Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

Depositor

Matthias Heinemann

Publication

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

S000001911 (TPS2, GSY1 Budding Yeast)

Expression

Yeast

Promoter

SNR52

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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