165,904 results

1. pET28a-Cas9-His

   Plasmid

   #98158

   Purpose

   Purification of Cas9 protein

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

   Insert

   NLS-Cas9-NLS
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AB-GFP

   Plasmid

   #72203

   Purpose

   screen AB42 variants for aggregation

   Depositor

   Michael Hecht

   Article

   Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

   Insert

   AB42 (APP Human)
   

   Tags

   GFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. NanoLuc-PM-Venus

   Plasmid

   #164786

   Purpose

   Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
   

   Tags

   NanoLuc and mVenus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

   Plasmid

   #71294

   Purpose

   Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Tags

   GNSTM glycosylation motif, HA, Lamp2 signal pepti…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_DKK1_WT

   Plasmid

   #82250

   Purpose

   Gateway Donor vector containing DKK1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DKK1 (DKK1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126706

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

   Insert

   ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
   

   Use

   CRISPR

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. piggyBAC-U6-GFP-WPRE

   Plasmid

   #200030

   Purpose

   piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

   Depositor

   Jay Shendure

   Type

   Empty backbone

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmEGFP-C1

   Plasmid

   #36412

   Depositor

   Benjamin Glick

   Type

   Empty backbone

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. 460 p3E-2A-mCherrypA

   Plasmid

   #195970

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-mCherry
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lifeact-3xmNG::HIS3

    Plasmid

    #227596

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::HIS3
    

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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