165,904 results

1. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

   Plasmid

   #162378

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE-Fast-FT

   Plasmid

   #31913

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Fast-FT
   

   Expression

   Mammalian

   Mutation

   M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

   Promoter

   PTight Tet-responsive PminCMVd

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCVpuro-PCAF

   Plasmid

   #63705

   Purpose

   retroviral expression of human PCAF in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   PCAF (KAT2B Human)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   E452G

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF1973

   Plasmid

   #142519

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TBX3 (TBX3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/cyto-iAspSnFR

   Plasmid

   #201394

   Purpose

   CMV overexpression of cyto-iAspSnFR in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

   Insert

   cyto-iAspSnFR
   

   Expression

   Mammalian

   Mutation

   gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

   Promoter

   CMV

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SV40-eGFP-Z1

   Plasmid

   #127638

   Purpose

   Minimal backbone plasmid for mammalian eGFP expression from SV40 promoter with zeocin/bleocin bacterial resistance

   Depositor

   Jordan Green

   Insert

   eGFP
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSimpleII-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47867

   Purpose

   This plasmid encode NmCas9 with two NLS and a HA tag

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Insert

   NmCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA flag PPAR gamma

   Plasmid

   #8895

   Purpose

   Mammalian expression of flag-tagged PPAR gamma

   Depositor

   Bruce Spiegelman

   Article

   Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

   Insert

   PPAR gamma (Pparg Mouse)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. Td-CBE-NW

   Plasmid

   #240623

   Purpose

   Cytidine base editor with narrowed editing window

   Depositor

   Tingting Jiang

   Article

   Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

   Insert

   TadA-8e (E27R, N46L, N108Q, M151C, Q154Y)-linker-nCas9-P2A-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   TadA-8e (E27R, N46L, N108Q, M151C, Q154Y), Cas9-P…

   Available Since

   July 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. AB.pCCL.sin.cPPT.GFP.miR-218-2-3p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85881

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-218-2-3p Sensor (MIR218-2 Human)
    

    Use

    Lentiviral

    Promoter

    PGK

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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