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Showing: 4201 - 4240 of 8321 results

  1. pAGM4723:Ble:Cas9

    Plasmid
    #112208
    Purpose
    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.
    Depositor
    Thomas Mock
    Inserts
    Cas9
    SheBle
    Use
    CRISPR; Diatom, phaeodactylum
    Tags
    Phaeodactylum fcp promoter and terminator and YFP
    Expression
    Mutation
    Phaeodactylum Fcp promoter
    Promoter
    Phaeodactylum Fcp
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112208-plasmid-map-sequence-id-217248

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  2. SIRT6

    Plasmid
    #41564
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT6 (SIRT6 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    aa 3-318
    Promoter
    T7
    Available since
    April 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. pYFP-NEAT1pr_v1-RT

    Plasmid
    #97087
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v1 vector, but also works with px330-NEAT1pr_v2.
    Depositor
    Archa Fox
    Insert
    nuclear paraspeckle assembly transcript 1 (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Expression
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97087-plasmid-map-sequence-id-189642

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  4. PTPN4

    Plasmid
    #38828
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN4 (Ptpn4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid
    #121170
    Purpose
    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    ABEmax-P2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    P2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121170-plasmid-map-sequence-id-234985

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  6. p3XFlag-S2P

    Plasmid
    #32966
    Purpose
    For mammalian expression of human S2P with a 3x FLAG tag
    Depositor
    Ron Prywes
    Insert
    S2P (MBTPS2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  7. pDGB3_alpha1_35S-eGFP-T35S (GB4279)

    Plasmid
    #225434
    Purpose
    Transcriptional unit for constitutive expression of the enhanced GFP.
    Depositor
    Diego Orzaez
    Insert
    35S:eGFP:T35S
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225434-plasmid-map-sequence-id-447741

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  8. tol2-4xnr UAS-PA Rac1-mCherry

    Plasmid
    #41878
    Depositor
    Anna Huttenlocher
    Insert
    photoactivatable Rac1 (RAC1 Human)
    Use
    Zebrafish expression
    Tags
    6x His and mCherry
    Expression
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    Available since
    Oct. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    41878-plasmid-map-sequence-id-56671

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  9. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72391-plasmid-map-sequence-id-133988

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  10. R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid
    #180153
    Purpose
    td-sfGFP dre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Mouse Targeting; Dre/rox
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180153-plasmid-map-sequence-id-355318

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  11. pcDNA3.3_SARS2_BA.2.75.2

    Plasmid
    #194498
    Purpose
    expressing SARS-CoV-2 BA2.75.2 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (BA.2.75.2) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R346T, F486S, D1199N and c-terminal 18aa del on t…
    Promoter
    CMV
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194498-plasmid-map-sequence-id-381635

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  12. pLVX-TIGHT-CYCLINF-Tet-OFF

    Plasmid
    #32977
    Depositor
    Michele Pagano
    Insert
    CYCLIN F (CCNF Human)
    Use
    Lentiviral
    Tags
    Flag and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    P-TIGHT
    Available since
    Feb. 13, 2012
    Availability
    Academic Institutions and Nonprofits only

  13. pINDUCER20-hMYOG-3xHA (#733)

    Plasmid
    #78342
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYOG ORF with a 3xHA tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYOG (MYOG Human)
    Use
    Lentiviral
    Tags
    3x HA tag
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. YWHAG

    Plasmid
    #39129
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    YWHAG (YWHAG Human)
    Use
    Tags
    Tev His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid
    #196722
    Purpose
    Tet-inducible expression of Cas9-T2A-miRFP670nano
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    TRE, PGK
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196722-plasmid-map-sequence-id-389804

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  16. PANK3

    Plasmid
    #32872
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK3 (PANK3 Human)
    Use
    Tags
    His tag (with Thrombin cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 10-370 only
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pTF272 (pFA6a-TEV-6xGly-3xFlag-HphMX)

    Plasmid
    #44083
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44083-plasmid-map-sequence-id-61676

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  18. pVimentin-Spot-Tag

    Plasmid
    #117059
    Purpose
    For mammalian expression of vimentin with C-terminal Spot-Tag
    Depositor
    Felix Hartlepp
    Insert
    vimentin (VIM Human)
    Use
    Tags
    Spot-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117059-plasmid-map-sequence-id-224393

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  19. pLenti.Syn(0.5).H2B-eGFP.W

    Plasmid
    #51004
    Purpose
    Lentivector that expresses H2B-eGFP from neurons-specific synapsin promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-EGFP (H2BC11 Synthetic, Jellyfish, Human)
    Use
    Lentiviral
    Tags
    histone2B
    Expression
    Mammalian
    Mutation
    Promoter
    rat synapsin
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51004-plasmid-map-sequence-id-130655

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  20. FVIII H2

    Plasmid
    #46773
    Purpose
    pcDNA4.full length FVIII D1241E
    Depositor
    Robert Peters
    Insert
    full length FVIII D1241E (F8 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D1241E
    Promoter
    CMV
    Available since
    Nov. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    46773-plasmid-map-sequence-id-66265

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  21. pDS-Red1-N1 AMPA GluR-B(flip)

    Plasmid
    #109142
    Purpose
    The expression vector carries the red fluorescent protein tagged at the C-terminus of GluA2 subunit flip spliced form of the AMPA receptor.
    Depositor
    Stefano Vicini
    Insert
    AMPA receptor
    Use
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    Glua2 subunit flip spliced form of the AMPA recep…
    Promoter
    CMV
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    109142-plasmid-map-sequence-id-209556

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  22. RAB3B (3DZ8)

    Plasmid
    #32879
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB3B (RAB3B Human)
    Use
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 18-190 only
    Promoter
    Available since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pMT-TRAF6-V5-His

    Plasmid
    #63752
    Purpose
    Expresses drosophila TRAF6 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    TRAF6 (Traf6 Fly)
    Use
    Tags
    V5-His
    Expression
    Insect
    Mutation
    Promoter
    pMT
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    63752-plasmid-map-sequence-id-106437

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  25. mEmerald-ALK5-N-13

    Plasmid
    #62751
    Purpose
    Localization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    ALK5 (TGFBR1 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. HaloTag-G protein gamma-12

    Plasmid
    #67043
    Purpose
    G protein gamma-12 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein gamma-12 (GNG12 Human)
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    67043-plasmid-map-sequence-id-115589

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  27. pZC13 (pFA6a-TEV-6xGly-V5-HphMX)

    Plasmid
    #44085
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44085-plasmid-map-sequence-id-61696

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  28. pActin5c-CD-dCas9m4-UDI-attB

    Plasmid
    #104879
    Purpose
    To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9
    Depositor
    Christopher Potter
    Insert
    CD-dCas9(m4)-UDI
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    104879-plasmid-map-sequence-id-209447

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  29. pEN_TT CXADR-V5

    Plasmid
    #220358
    Purpose
    Gateway entry vector for an inducible V5-tagged CXADR
    Depositor
    Kevin Janes
    Insert
    CXADR (CXADR Human)
    Use
    Entry vector for gateway cloning
    Tags
    V5
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220358-plasmid-map-sequence-id-441247

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  30. PAS_RvSAHS6

    Plasmid
    #215647
    Purpose
    Expresses SUMO-tagged RvSAHS6 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS6
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215647-plasmid-map-sequence-id-426094

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  31. PAS_RvSAHS1

    Plasmid
    #215645
    Purpose
    Expresses SUMO-tagged RvSAHS1 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS1
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215645-plasmid-map-sequence-id-426092

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  32. SRPK2

    Plasmid
    #39047
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SRPK2 (SRPK2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Internal segment 268-518 deleted; codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. pAG426-GAL-UBQLN2

    Plasmid
    #181708
    Purpose
    Galactose inducible expression of UBQLN2
    Depositor
    Alejandro Chavez
    Insert
    UBQLN2 (UBQLN2 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    181708-plasmid-map-sequence-id-436183

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  34. pcDNA3.1 Flag-Eya2

    Plasmid
    #49264
    Purpose
    expresses Flag tagged Eya2
    Depositor
    Heide Ford
    Insert
    Eya2 (EYA2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. pLEX307-CTSL-puro

    Plasmid
    #158466
    Purpose
    Constitutive lentiviral expression of human CTSL.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSL (CTSL Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158466-plasmid-map-sequence-id-308153

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  36. pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)

    Plasmid
    #44095
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44095-plasmid-map-sequence-id-61672

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  37. pcDNA3.1_RapACR_T111C_N248Q-EYFP

    Plasmid
    #119197
    Purpose
    Mammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248Q
    Depositor
    John Spudich
    Insert
    Anion channelrhodopsin RapACR fast mutant T111C_N248Q
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    changed threonine 111 to cysteine and asparagine …
    Promoter
    CMV
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    119197-plasmid-map-sequence-id-230387

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  38. pCeru-N1 cyclin A (1557)

    Plasmid
    #61842
    Purpose
    mammalian expression of cyclin A with cerulean fusion
    Depositor
    Jonathon Pines
    Insert
    cyclin A (CCNA2 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pAAV-Dlx5/6-Chronos-eGFP-p2a-nls-mScarlet

    Plasmid
    #121543
    Purpose
    Chronos with red nucleus-targeted fluorescence for inhibitory neurons
    Depositor
    Matthias Prigge
    Insert
    blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    dlx5/6
    Available since
    Feb. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    121543-plasmid-map-sequence-id-235975

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  40. pCI-ING3

    Plasmid
    #79070
    Purpose
    Expression of p47 ING3 in mammalian cells
    Depositor
    Karl Riabowol
    Insert
    ING3 (p47 ING3 isoform) (ING3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 4201 - 4240 of 8321 results