We narrowed to 8,862 results for: BLI;
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CAPZA1-TwinStrepII
Plasmid#83193PurposeExpression of tagged protein in mammalian cellsDepositorInsertCAPZA1 (Capza1 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS
Plasmid#191527PurposeDonor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locusDepositorInserthGEM(1-110)_mNG_hSLBP(18-108)
UseCRISPR and Synthetic BiologyTagsmNeonGreen (mNG) + 3xFLAG_TwinStrepExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCLIPf-NK1R Control Plasmid
Plasmid#101125PurposeExpression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cellsDepositorInsertNeurokinin-1 Receptor, Truncated (TACR1 Human)
UseTags10xHis, CLIP-tag (CLIPf), and FLAG EpitopeExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103 BAG3 WW domain
Plasmid#104266PurposeBacterial expression of WW domain from BAG3DepositorInsertBAG3 WW domain (BAG3 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALPS-ZsGreen-miR-146a
Plasmid#89174Purposeencodes human/murine miR-146a and ZsGreen1DepositorUseLentiviralTagsExpressionMutationPromoterSFFVpAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IV-3xFLAG/HA/NBL10
Plasmid#110568PurposeCre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tagDepositorInsert3xFLAG/HA-tagged anti-GFP VHH
UseAAV, Cre/Lox, and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCXLE_hePOU_shp53
Plasmid#206397PurposeIntegration-free (episomal) expression of human ePOU and shRNA against p53.DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Galectin-C-18
Plasmid#62745PurposeLocalization: Membrane Receptor, C1 Cloning Vector, Excitation:587, Emission:610DepositorInsertGalectin (LGALS1 Human)
UseTagsmCherryExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT-Flag-RELISH
Plasmid#63750PurposeExpresses drosophila RELISH tagged with a Flag epitopes, under control of metallothionein promoterDepositorInsertRelish (Rel Fly)
UseTagsFlagExpressionInsectMutationPromoterpMTAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIC144
Plasmid#63067PurposeRetroviral expression of EGFP-TEV-S-tag CENP-K for protein localization and affinity purification (LAP)DepositorInsertCENP-K, IMAGE clone 3511065 (CENPK Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGFP CrkII
Plasmid#50730Purposemammalian expression of GFP-CrkII. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertCrkII (CRK Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pEF-GalNAcT2-moxGFP2
Plasmid#218966PurposeExpresses full length GalNAcT2-moxGFP2 mammalian cellsDepositorInsertGalNAcT2-moxGFP2 (GALNT2 Human)
UseTagsmoxGFP2ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHA_OROV_NSm
Plasmid#208940PurposeExpresses C-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cellsDepositorInsertNSm
UseTagsHAExpressionMammalianMutationDeleted 949 bp from 5' end (Gn), deleted 29…PromoterCMVAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNHA_OROV_NSm
Plasmid#208935PurposeExpresses N-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cellsDepositorInsertNSm
UseTagsHAExpressionMammalianMutationDeleted 949 bp from 5' end (Gn), deleted 29…PromoterCMVAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV Cbx1-V5
Plasmid#175106PurposeLentiviral expression of mouse Cbx1-V5DepositorInsertCbx1 (Cbx1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV hMyoD-IRES-dsRed
Plasmid#66631PurposeCo-expresses human MyoD and dsRedDepositorInserthuman MyoD IRES dsRed (MYOD1 Human)
UseLentiviralTagsExpressionMutationPromoterhUbcAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-TMPRSS11D-puro
Plasmid#158475PurposeConstitutive lentiviral expression of human TMPRSS11D.DepositorInsertTMPRSS11D (TMPRSS11D Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast shLuc
Plasmid#110409PurposeshLuc (Target TTACGCTGAGTACTTCGA), silence LUC gene, blasticidin selection.DepositorInsertLuciferase
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA PolIII)Available sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-MCAK-C-7
Plasmid#62785PurposeLocalization: Microtubules/Kinesin, C1 Cloning Vector, Excitation:399, Emission:456DepositorInsertMCAK (KIF2C Human)
UseTagsmTagBFP2ExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMB018 / LRRK2_BC_1333_2187
Plasmid#40510DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTags6xHisExpressionMutationWT; aa1333_2187PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
STXBP1 in pENTR
Plasmid#216624Purposegateway cloning donor vector containing CTNNB1 ASD mutationDepositorInsertSTXBP1 (STXBP1 Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pUASp YFP Ras64B DN
Plasmid#52243PurposeDrosophila expression of YFP-Ras64B dominant negative mutantDepositorInsertRas64B (Ras64B Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS19N; DN mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras64B CA
Plasmid#52244PurposeDrosophila expression of YFP-Ras64B constitutively active mutantDepositorInsertRas64B (Ras64B Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ63L; CA mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRlsinPGK_dectintagBFP2_WPRE
Plasmid#91786PurposeConstitutive expression of mTagBFP2-Dectin1A-N-10 using PGK promoter in a lentiviral backbone.DepositorInsertDectin1A (CLEC7A Human)
UseLentiviralTagsmTagBFP2ExpressionMammalianMutationPromoterPGKAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2Lox
Plasmid#34635DepositorTypeEmpty backboneUseTagsEGFPExpressionBacterialMutationPromoterPGK promoterAvailable sinceFeb. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-C3-Cep123
Plasmid#73330PurposeLentiviral vector encoding Cep123 isoform 1 with an N-terminal EGFP tagDepositorInsertCep123 (CEP89 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSpot-Tag-Actin
Plasmid#117060PurposeFor mammalian expression of beta-actin with N-terminal Spot-TagDepositorInsertactin beta (ACTB Human)
UseTagsSpot-TagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXA5 (human) HIS-tag pET
Plasmid#8562DepositorInsertHOXA5 (HOXA5 Human)
UseTagsHis and T7ExpressionBacterialMutationSacI site at amino acids 1-2 was used to clone in…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIC80
Plasmid#63064PurposeMammalian expression of EGFP-TEV-S-tag DSN1 for protein localization and affinity purification (LAP)DepositorInsertDSN1, IMAGE clone 6063417 (DSN1 Human)
UseTagsEYFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV Synapsin Intron H2B Gcamp 6f wpre Pzac2.1
Plasmid#74144PurposeCalcium sensorDepositorInsertGCaMP6f
UseAAVTagsH2BExpressionMutationPromotersynapsinAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pGEX6P1-N-Myc
Plasmid#119757PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.DepositorTypeEmpty backboneUseTagsGST and MycExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
4QPP
Plasmid#61964PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertHRMT1L6:PBC001-A12:C213642 (PRMT6 Human)
UseBaculovirus expressionTags6x His and TEV cleavage siteExpressionMutationSee commentsPromoterPolyhedrinAvailable sinceSept. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAL-c5X-35-H-6
Plasmid#67008PurposeExpresses malE-35-H-6 in E. coliDepositorInsertmalE-35-H-6
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
APEX2 VLP
Plasmid#182211PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).DepositorInsertsVP2C-APEX2
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic Biology; Tem imagingTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-zeo-hFJX1-myc
Plasmid#46930Purposeexpression of human FJX1-myc in mammalian cellsDepositorInsertFJX1 (FJX1 Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pAS_MmaPylT_EF1_NES-MmaPylRS AF
Plasmid#174902Purposeexpression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAG and NESExpressionMammalianMutationY306A, Y384FPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
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AAV Ef1a-mRuby3-WPRE
Plasmid#135427PurposeCan be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.DepositorInsertmRuby3
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mouse-Dysferlin-Transcript-Variant2
Plasmid#60215PurposeMouse dysferlin transcript variant-2 (accession #NM_001077694) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.DepositorInsertDysferlin (Dysf Mouse)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
4QQ6
Plasmid#61946PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSMN1:JMC047-F11:C221089 (SMN1 Human)
UseTags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
