We narrowed to 2,171 results for: Xpa
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Plasmid#231129PurposeExpresses mutant E. coli TrpRS from a weak glnS promoter for proteome-wide incorporation of tryptophan analogues.DepositorInsertE. coli tryptophanyl tRNA synthetase
ExpressionBacterialMutationS8A, V144G, V146CAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-EcTrpRS(H14)
Plasmid#231131PurposeExpresses mutant E. coli TrpRS from a derepressed lac promoter for proteome-wide incorporation of tryptophan analogues in E. coli/K. pneumoniae.DepositorInsertE. coli tryptophanyl tRNA synthetase
UseKlebsiella pneumoniae expressionExpressionBacterialMutationS8A, V144G, V146CAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSGAb/pEvol-AbTrpRS(H14)
Plasmid#231133PurposeExpresses mutant A. baumannii TrpRS from an inducible lac promoter for proteome-wide incorporation of tryptophan analogues.DepositorInsertA. baumannii tryptophanyl tRNA synthetase
UseAcinetobacter baumannii expressionExpressionBacterialMutationT12A, V151G, V153CAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7CNW)RS
Plasmid#222874PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(5CNW)RS
Plasmid#222872PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr
Plasmid#218766Purposeencodes EcTyrRS-VSMA* and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-VSMA*
ExpressionBacterialMutationY37V, D167G, D182S, F183M, L186A, D265RPromotertacIAvailable SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.2.t1
Plasmid#73546PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.2.t1
pAzFRS.2.t1
ExpressionBacterialAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAcFRS.2.t1
Plasmid#73544PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.2.t1
pAcFRS.2.t1
ExpressionBacterialAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_FLAG-Mx PylRS
Plasmid#140011PurposePlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianPromoterEF1Available SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPlin5-mScarlet3-H (aka mYongHong)
Plasmid#239408PurposeExpresses Plin5-mScarlet3-H in mammalian cellsDepositorInsertPlin5 (PLIN5 Human)
TagsmScarlet3-H (aka mYongHong)ExpressionMammalianMutationM163H in mScarlet3PromoterCMVAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMaRSC
Plasmid#89189PurposeThe pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.DepositorInsertFlag-L274G-T2A-mCherry (Mars1 Mouse)
Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
polyQ74-GFP
Plasmid#231893PurposeForms cytosolic HTT partial exon 1 Q74 aggregatesDepositorInsertHTT partial exon 1 Q74 (HTT Human)
TagsEGFPExpressionMammalianMutationCAG repeat expansionAvailable SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDule-Tet2.0
Plasmid#85496PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.1.t1
Plasmid#73547PurposetRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.1.t1
pAzFRS.1.t1
ExpressionBacterialAvailable SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-ABK
Plasmid#126035PurposeEfficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.DepositorInsertstRNA synthetase 1
tRNA synthetase 2
tRNA for TAG codon
ExpressionBacterialMutationL274M, C313A, Y349FPromoteraraBAD promoter, glnS promoter, and proK promoterAvailable SinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBpaRS3tRNA
Plasmid#197102PurposeTo express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.DepositorInsertTyrRS variant, amber suppressor tRNA
ExpressionBacterialMutationGly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAcBac1.tR4-AcdRS82
Plasmid#174101PurposeExpression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.DepositorInsertM. barkeri tRNA synthetase for acridone82
ExpressionMammalianMutationN311S, C313G, V366A, W382TPromoterCMVAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDule-3-nitroTyrosine (5B)
Plasmid#85498PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGLACTE-PseudoHT51
Plasmid#99257PurposeContains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresisDepositorInsertFMR1 (FMR1 Human, Synthetic)
MutationContains synthetic, non-human, primer sequence do…Available SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only