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We narrowed to 4,156 results for: pCMV mammalian

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Showing: 2101 - 2120 of 4156 results

  1. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

    Plasmid
    #139991
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139991-plasmid-map-sequence-id-275523

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  3. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

    Plasmid
    #139995
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    HF1=N497A/R661A/Q695A/Q926A
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139995-plasmid-map-sequence-id-275425

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  6. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid
    #174136
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174136-plasmid-map-sequence-id-341914

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  12. pCMV-MMLV-gag-hMLH1dn

    Plasmid
    #195513
    Purpose
    MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.
    Depositor
    Hyongbum Kim
    Article
    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.
    Insert
    hMLH1dn (MLH1 Human)
    Use
    Virus like particle
    Tags
    FLAG, NES, and Protease cleavate site
    Expression
    Mammalian
    Mutation
    Human MLH1 del754-756
    Promoter
    CMV
    Available Since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    195513-plasmid-map-sequence-id-384690

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Showing: 2101 - 2120 of 4156 results