We narrowed to 6,558 results for: sas

1. pD649-HAsp-SEMA3E-COMP5AP-AviTag-9xHis

   Plasmid

   #157534

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA3E (SEMA3E Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD649-HAsp-SIGIRR-COMP5AP-AviTag-9xHis

   Plasmid

   #157058

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SIGIRR (SIGIRR Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSET.GltI253-cpGFP.L1LV/L2NP

   Plasmid

   #41733

   Purpose

   a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Insert

   GltI based Glutamate Biosensor
   

   Use

   Glutamate biosensor

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

   Promoter

   T7

   Available Since

   Jan. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJet-CMV-IARS1[W435C]-GFP

   Plasmid

   #212323

   Purpose

   EGFP-tagged IARS1 Trp435Cys

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   IARS (IARS1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Trp435cys

   Promoter

   CMV

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-ptetO::bac::gfp

   Plasmid

   #217882

   Purpose

   expresses the bac-BGC in bacterial cells, produces bacillamide D

   Depositor

   Tobias A. M. Gulder

   Article

   Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

   Inserts

   Dehydrogenase
   Non-ribosomal peptide synthetase
   Aminotransferase
   

   Expression

   Bacterial

   Promoter

   ptetO

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-Bhlhe40-3xFLAG-IRES-BFP

   Plasmid

   #117263

   Purpose

   Retroviral overexpression of Bhlhe40 with a 3xFLAG

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   Bhlhe40 (Bhlhe40 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTT3-SP-6xHis-KIR2DL5(FL)-FLAG

   Plasmid

   #157623

   Purpose

   Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR2DL5 (KIR2DL5A Human)
   

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-CD1B-COMP5AP-AviTag-9xHis

   Plasmid

   #157404

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CD1B (CD1B Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nxph4-2A-CreERT2 Targeting Vector

   Plasmid

   #62219

   Purpose

   Targets CreERT2 to the mouse Nxph4 stop codon

   Depositor

   Hongkui Zeng

   Article

   Harris et al Front Neural Circuits. 2014 Jul 10;8:76.

   Insert

   Nxph4-2A-CreERT2 (Nxph4 Bacteriophage, Mouse)
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Promoter

   Mouse Nxph4

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}

    Plasmid

    #201120

    Purpose

    RFP expression in cells under EF-1α promoter; Histone-bound, nuclear

    Depositor

    Ori Bar-Nur

    Article

    Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

    Insert

    TurboRFP; H2B-RFP
    

    Use

    Lentiviral

    Mutation

    No

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-mCherry-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156581

    Purpose

    Mammalian expression of mCherry fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    mCherry
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO_HA.V5.FLAG_NGFR

    Plasmid

    #158250

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.V5.FLAG

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-KIR2DS1-COMP5AP-AviTag-9xHis

    Plasmid

    #157239

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR2DS1 (KIR2DS1 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO_Ollas.S.V5_mCherry-NLS

    Plasmid

    #178211

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    Nuclear Localization Signal and Ollas.S.V5

    Promoter

    eF1a

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pD649-HAsp-NPHS1-COMP5AP-AviTag-9xHis

    Plasmid

    #157587

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NPHS1 (NPHS1 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5 FLAG 17xS/T->A, catalytic active lipin 1

    Plasmid

    #32007

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S106A, S150A, (S281A, T282A), S285A, S287A, S293A…

    Promoter

    CMV, SP6

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pAR100

    Plasmid

    #180377

    Purpose

    pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).

    Depositor

    Alec Redwood

    Article

    Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:

    Inserts

    NFAT-Luciferase
    hCD8A
    

    Expression

    Bacterial and Mammalian

    Promoter

    hEF1-HTLV and minimal promoter and an NFAT respon…

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pD649-HAsp-ROBO1-COMP5AP-AviTag-9xHis

    Plasmid

    #157562

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    ROBO1 (ROBO1 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pD649-HAsp-NEO1-COMP5AP-AviTag-9xHis

    Plasmid

    #157594

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NEO1 (NEO1 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV_CMV_CCR6-Nanoluc-V5

    Plasmid

    #199672

    Purpose

    expresses CCR6-NanoLuc-V5 in mammalian cells, AAV vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    CCR6 (CCR6 Human)
    

    Use

    AAV

    Tags

    NanoLuc and V5

    Expression

    Mammalian

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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