We narrowed to 4,186 results for: GCA

1. shLacZ

   Plasmid

   #42559

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shLacZ
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

2. gh38

   Plasmid

   #106712

   Purpose

   expression of gRNA targeting STT3A

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   STT3A (STT3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-gRNA-puro

   Plasmid

   #180426

   Purpose

   Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

   Insert

   gRNAscr
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GOLPH3 shRNA

   Plasmid

   #163860

   Purpose

   shRNA targeting the coding sequences of GOLPH3

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   golgi phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLentiCRISPRv2-dADAM17 (#1)

   Plasmid

   #177259

   Purpose

   A knockout vector for the dog Adam17

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   A gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLKO-STAG2 shRNA 1221

   Plasmid

   #31978

   Depositor

   Todd Waldman

   Article

   Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

   Insert

   STAG2 (STAG2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pSUPERIORpuro-shGAS5

   Plasmid

   #46370

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

   Insert

   GAS5 (GAS5 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1

   Available since

   Aug. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pSUPER IKKbeta

   Plasmid

   #26209

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   pSUPER IKK beta (IKBKB Human)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pLKO-puro-sh-mFAK B

   Plasmid

   #37018

   Depositor

   Bob Weinberg

   Article

   Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

   Insert

   sh-mFAK B (Ptk2 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 31, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pTorPE-G-GECO1.2

    Plasmid

    #32468

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.2
    

    Use

    Lab constructed

    Tags

    6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

    Expression

    Bacterial

    Mutation

    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. px330-UFL1 sgRNA2

    Plasmid

    #134636

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA2 (UFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEY12

    Plasmid

    #191018

    Purpose

    Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    rab-3 (rab-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEY13

    Plasmid

    #191019

    Purpose

    Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    arrd-4 (ZK938.2 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSMP-G9A_4

    Plasmid

    #36334

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    G9A (EHMT2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pDECKO_TFRC_B

    Plasmid

    #72626

    Purpose

    Expresses two gRNAs targeting the TFRC promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward TFRC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiCRISPRv2 sgSp1

    Plasmid

    #174907

    Purpose

    Second generation sgRNA against Sp1

    Depositor

    Jane Clifford

    Article

    Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

    Insert

    Specificity Protein 1 (SP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Missense mutation to mutate PAM sequence

    Promoter

    EFS-NS

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTX209

    Plasmid

    #89269

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsPDS-gRNA01 and OsPDS-gRNA02
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Maize Ubiquitin1 promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psiCHECK2-miR-34 MT

    Plasmid

    #78259

    Purpose

    psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    mutated miR-34 target site
    

    Use

    Luciferase; Microrna activity

    Tags

    Expression

    Mammalian

    Mutation

    nucleotides 2-5 and 10-11 changed from CCGT to GG…

    Promoter

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pgRNA-non-target

    Plasmid

    #129465

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Non-target/random gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO-puro human lipin 1-1 shRNA

    Plasmid

    #32019

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only