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Showing: 2121 - 2140 of 3086 results

  1. pGLO-Magenta-AZA

    Plasmid
    #220784
    Purpose
    Expresses a magenta chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)
    Depositor
    Angela Leu
    Insert
    magenta chromoprotein
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220784-plasmid-map-sequence-id-437914

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  2. CRISPR-Sa-TdTom. Reporter

    Plasmid
    #99652
    Purpose
    Red fluorescent reporter that can be activated by dSa Cas9 activator indicating activity
    Depositor
    George Church
    Insert
    RFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    99652-plasmid-map-sequence-id-192053

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  3. pKT-H3.3-K27M-katushka

    Plasmid
    #209443
    Purpose
    SB-transposable plasmid expressing a mutant histone H3.3-K27M in tandem with Katushka (RFP)
    Depositor
    Maria Castro
    Insert
    H3.3-K27M
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K27M
    Promoter
    Available since
    Jan. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    209443-plasmid-map-sequence-id-418361

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  4. pKT-H3.3-G34R-IRES-katushka

    Plasmid
    #209428
    Purpose
    SB-transposable plasmid expressing the mutant histone H3.3-G34R with RFP
    Depositor
    Maria Castro
    Insert
    H3.3-G34R
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G34R
    Promoter
    Available since
    Jan. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    209428-plasmid-map-sequence-id-418354

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  5. hPLD3-sgRNA-Cas9_mcherry

    Plasmid
    #199342
    Purpose
    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199342-plasmid-map-sequence-id-390619

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  6. mPLD3-sgRNA-Cas9-mcherry

    Plasmid
    #199700
    Purpose
    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199700-plasmid-map-sequence-id-390617

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  7. yeast_DualReporter

    Plasmid
    #127546
    Purpose
    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
    Depositor
    Aviv Regev
    Article
    de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do
    Inserts
    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127546-plasmid-map-sequence-id-251803

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  8. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

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  9. pDS-Red-N1 GABAA beta3

    Plasmid
    #109143
    Purpose
    The expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.
    Depositor
    Stefano Vicini
    Insert
    GABAa receptor
    Use
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    beta3 subunit of the GABAa receptor
    Promoter
    CMV
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    109143-plasmid-map-sequence-id-210126

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  10. pBFC0993

    Plasmid
    #231993
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and pTet
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231993-plasmid-map-sequence-id-467616

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  11. pSBBi-RP CCS1

    Plasmid
    #139162
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    caffeine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139162-plasmid-map-sequence-id-309352

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  12. pSBBi-RP CsAncCS

    Plasmid
    #139167
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Camellia sinensis ancestral caffeine synthase (CsAncCS); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    Predicted Camellia sinensis Ancestral Caffeine Synthase
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139167-plasmid-map-sequence-id-309357

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  13. pSBBi-RP CaMXMT

    Plasmid
    #139172
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica monomethylxanthine methyltransferase (CaMXMT); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    monomethylxanthine methyltransferase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139172-plasmid-map-sequence-id-309362

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  14. pDS-Red1-N1 AMPA GluR-B(flip)

    Plasmid
    #109142
    Purpose
    The expression vector carries the red fluorescent protein tagged at the C-terminus of GluA2 subunit flip spliced form of the AMPA receptor.
    Depositor
    Stefano Vicini
    Insert
    AMPA receptor
    Use
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    Glua2 subunit flip spliced form of the AMPA recep…
    Promoter
    CMV
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    109142-plasmid-map-sequence-id-209556

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  15. pSBBi-RP PcCS

    Plasmid
    #139159
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana caffeine synthase (PcCS); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    Caffeine Synthase [Paullinia cupana var. sorbilis]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139159-plasmid-map-sequence-id-309125

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  16. pSBBi-RP TCS1

    Plasmid
    #139161
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    Caffeine Synthase 1 [Camellia sinensis]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139161-plasmid-map-sequence-id-309127

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  17. pSBBi-RP CaDXMT

    Plasmid
    #139165
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffee arabica dimethylxanthine methyl transferase (CaDXMT) ; the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    3,7-dimethylxanthine N-methyltransferase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139165-plasmid-map-sequence-id-309355

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  18. pSBBi-RP PcAncCS2

    Plasmid
    #139170
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana ancestral Caffeine Synthase 2 (PcAncCS2); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    Predicted Paullinia cupana var sobilis Ancenstral Caffeine Synthase-2
    Use
    Transposon
    Tags
    None
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139170-plasmid-map-sequence-id-309360

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  20. pSG10-pGlnA-mCherry

    Plasmid
    #102467
    Purpose
    Expresses mCherry fluorescent protein in E. coli. The expression is controlled by a pGlnA promoter.
    Depositor
    Richard Murray
    Article
    Guo et al bioRxiv 122606
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pGlnA
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    102467-plasmid-map-sequence-id-200305

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Showing: 2121 - 2140 of 3086 results