Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 8,624 results for: FIE

Showing: 4241 - 4280 of 8624 results

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pUb FLAG-Mts

Plasmid

#195065

Purpose

Expresses FLAG-tagged Drosophila mts protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Pp2A-29B

Plasmid

#195066

Purpose

Expresses FLAG-tagged Drosophila Pp2A-29B protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Protein phosphatase 2A at 29B (Pp2A-29B Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-MVP delta607-623aa

Plasmid

#204542

Purpose

Expresses GST-tagged MVP delta607-623aa in E.coli

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Expression

Bacterial

Mutation

delta607-623aa

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-dMET

Plasmid

#176031

Purpose

A knock-out vector for dog MET

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog MET gene and the cDNA of Cas9 (MET )

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD-R133C

Plasmid

#179409

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R133C)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(R133C) (Mecp2 Mouse)

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R133C

Promoter

CAGGS

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD-T158M

Plasmid

#179410

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(T158M)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(T158M) (Mecp2 Mouse)

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD T158M

Promoter

CAGGS

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PMP34-hLOV-TEVcs-GAL4bd-VP64-V5

Plasmid

#170997

Purpose

HiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

PMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

V5

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_PSMD1_sgRNA_1

Plasmid

#155102

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting PSMD1 (core essential gene)

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

PSMD1_sgRNA_1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_AAVS1_sgRNA_4

Plasmid

#155101

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO_AAVS1_sgRNA_3

Plasmid

#155086

Purpose

lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_AAVS1_sgRNA_4

Plasmid

#155106

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO_AAVS1_sgRNA_4

Plasmid

#155087

Purpose

lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_AAVS1_sgRNA_4

Plasmid

#155090

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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AAV2_hSyn_Aurora_CA_Citrine

Plasmid

#98219

Purpose

Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

Depositor

Simon Wiegert

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Aurora_C128A gene

Use

AAV

Tags

Citrine

Expression

Mammalian

Mutation

V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

Promoter

human synapsin

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PSG2_WT

Plasmid

#81917

Purpose

Gateway Donor vector containing PSG2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PSG2 (PSG2 Human)

Use

Gateway entry vector

Mutation

V20L; p.333_335del-NPT-ins-IQH

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMR3A_WT

Plasmid

#81887

Purpose

Gateway Donor vector containing SMR3A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMR3A (SMR3A Human)

Use

Gateway entry vector

Mutation

C42R; P132L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_C1orf150_WT

Plasmid

#81842

Purpose

Gateway Donor vector containing C1ORF150 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C1ORF150 (GCSAML Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SPAG8_WT

Plasmid

#81848

Purpose

Gateway Donor vector containing SPAG8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPAG8 (SPAG8 Human)

Use

Gateway entry vector

Mutation

E2D; E5G; P226L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_CPXCR1_WT

Plasmid

#81819

Purpose

Gateway Donor vector containing CPXCR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CPXCR1 (CPXCR1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_OR2M2_WT

Plasmid

#81758

Purpose

Gateway Donor vector containing OR2M2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

R220G, C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_MTM1_p.R253Q

Plasmid

#81724

Purpose

Gateway Donor vector containing MTM1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MTM1 (MTM1 Human)

Use

Gateway entry vector

Mutation

R253Q; E410K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_MTM1_p.P226S

Plasmid

#81678

Purpose

Gateway Donor vector containing MTM1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MTM1 (MTM1 Human)

Use

Gateway entry vector

Mutation

P226S; E410K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_OR2M2_p.N42Y

Plasmid

#81638

Purpose

Gateway Donor vector containing OR2M2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

N42Y; R220G; C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_OR2M2_p.V117I

Plasmid

#81639

Purpose

Gateway Donor vector containing OR2M2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

V117I; R220G; C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_STK19_p.E88K

Plasmid

#81649

Purpose

Gateway Donor vector containing STK19, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STK19 (STK19 Human)

Use

Gateway entry vector

Mutation

E88K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_OR2M2_p.E232V

Plasmid

#81634

Purpose

Gateway Donor vector containing OR2M2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

R220G; E232V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_OR2M2_p.P182S

Plasmid

#81635

Purpose

Gateway Donor vector containing OR2M2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

P182S; R220G; C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_TUBB_p.R359P

Plasmid

#81617

Purpose

Gateway Donor vector containing TUBB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TUBB (TUBB Human)

Use

Gateway entry vector

Mutation

R359P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_MAGEC2_p.D15N

Plasmid

#81624

Purpose

Gateway Donor vector containing MAGEC2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAGEC2 (MAGEC2 Human)

Use

Gateway entry vector

Mutation

D15N; R262K; E373S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SERPINB4_p.E250K

Plasmid

#81614

Purpose

Gateway Donor vector containing SERPINB4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SERPINB4 (SERPINB4 Human)

Use

Gateway entry vector

Mutation

E250K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_PIK3CB_p.E497D

Plasmid

#81599

Purpose

Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway entry vector

Mutation

E497D; Q759R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_PIK3CB_p.A593V

Plasmid

#81602

Purpose

Gateway Donor vector containing PIK3CB ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway entry vector

Mutation

A593V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SYCP1_p.E442K

Plasmid

#81585

Purpose

Gateway Donor vector containing SYCP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SYCP1 (SYCP1 Human)

Use

Gateway entry vector

Mutation

E442K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SYCP1_p.E509K

Plasmid

#81586

Purpose

Gateway Donor vector containing SYCP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SYCP1 (SYCP1 Human)

Use

Gateway entry vector

Mutation

E509K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_TRAF3_p.N312S

Plasmid

#81574

Purpose

Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF3 (TRAF3 Human)

Use

Gateway entry vector

Mutation

K5R; N312S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_THEMIS_p.D240N

Plasmid

#81552

Purpose

Gateway Donor vector containing THEMIS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

THEMIS (THEMIS Human)

Use

Gateway entry vector

Mutation

D240N; I630V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_THEMIS_p.H366Q

Plasmid

#81560

Purpose

Gateway Donor vector containing THEMIS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

THEMIS (THEMIS Human)

Use

Gateway entry vector

Mutation

H366Q; I630V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_PIK3CB_p.A1048V

Plasmid

#81533

Purpose

Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway entry vector

Mutation

A1048V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SYCP1_p.E773K

Plasmid

#81535

Purpose

Gateway Donor vector containing SYCP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SYCP1 (SYCP1 Human)

Use

Gateway entry vector

Mutation

E773K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SYCP1_p.D889N

Plasmid

#81538

Purpose

Gateway Donor vector containing SYCP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SYCP1 (SYCP1 Human)

Use

Gateway entry vector

Mutation

D889N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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