Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 9,339 results for: UTY

Showing: 5301 - 5350 of 9339 results

  2. pGBT9-FHIP

    Plasmid
    #198527
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979
    Insert
    FHIP (FHIP1B Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    silent mutations in codons 353 and 651 as well as…
    Promoter
    ADH1
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198527-plasmid-map-sequence-id-393106

    Enlarge

  3. RAT +D122H

    Plasmid
    #187426
    Purpose
    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al bioRxiv 2021.11.29.470343
    Inserts
    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    Use
    Tags
    Expression
    Insect
    Mutation
    changed aspartic acid at 129 for histidine
    Promoter
    PH and p10
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    187426-plasmid-map-sequence-id-374214

    Enlarge

  4. RAT +R111E

    Plasmid
    #187425
    Purpose
    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al bioRxiv 2021.11.29.470343
    Inserts
    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    Use
    Tags
    Expression
    Insect
    Mutation
    changed arginine at 118 to glutamic acid
    Promoter
    PH and p10
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    187425-plasmid-map-sequence-id-374210

    Enlarge

  6. MSNA-c029

    Plasmid
    #175471
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44.
    Depositor
    Opher Gileadi
    Article
    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10
    Insert
    MSN (MSN Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    L281R, H288A
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175471-plasmid-map-sequence-id-353253

    Enlarge

  7. ST-Tstem(5A)-GFP

    Plasmid
    #162502
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The mutated Tac stem region is inserted within ST…
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    162502-plasmid-map-sequence-id-323507

    Enlarge

  8. GFP-Tac-STC(5A)

    Plasmid
    #162491
    Purpose
    Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its stem region, TM…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162491-plasmid-map-sequence-id-323469

    Enlarge

  9. pLCHKO_TK1_HPRT1_Lb_6

    Plasmid
    #155064
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155064-plasmid-map-sequence-id-308583

    Enlarge

  10. pLCHKO_TK1_HPRT1_Lb_5

    Plasmid
    #155063
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155063-plasmid-map-sequence-id-308581

    Enlarge

  11. pLCHKO_TK1_HPRT1_Lb_3

    Plasmid
    #155061
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155061-plasmid-map-sequence-id-308580

    Enlarge

  12. pLCHKO_TK1_HPRT1_Lb_2

    Plasmid
    #155060
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and an intergenic site & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155060-plasmid-map-sequence-id-308523

    Enlarge

  13. pDONR_P4-P1R:PER36modp

    Plasmid
    #128431
    Purpose
    Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).
    Depositor
    George Haughn
    Article
    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100
    Insert
    PEROXISADE36 (AT3G50990 Mustard Weed)
    Use
    Gateway promoter entry clone
    Tags
    Expression
    Mutation
    Premature stop codons introduced into the Xero1 (…
    Promoter
    PEROXISADE36
    Available since
    Oct. 4, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    128431-plasmid-map-sequence-id-257603

    Enlarge

  24. pLKO.1-TRC.mKO2_shHRASLS.2

    Plasmid
    #110324
    Purpose
    TRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    HRASLS (PLAAT1 Human, Homo sapiens)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110324-plasmid-map-sequence-id-213397

    Enlarge

  25. pLKO.1-TRC.mKO2_shHRASLS.1

    Plasmid
    #110323
    Purpose
    TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    HRASLS (PLAAT1 Human, Homo sapiens)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110323-plasmid-map-sequence-id-213396

    Enlarge

  26. pFUSEss-CHIg-mG3_VPEVS

    Plasmid
    #105863
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG3 heavy chain (Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutated heavy chain of mouse IgG3 with five mutat…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    105863-plasmid-map-sequence-id-206035

    Enlarge

  27. pFUSEss-CHIg-mG1_ILGGP

    Plasmid
    #105855
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1 heavy chain (Ighg1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutated heavy chain of mouse IgG1 with five mutat…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    105855-plasmid-map-sequence-id-206027

    Enlarge

  28. pFUSEss-CHIg-mG1_CH2charge

    Plasmid
    #105852
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutations modify charge of CH2 domain.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1 heavy chain (Ighg1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutated heavy chain of mouse IgG1, muatations: Gl…
    Promoter
    hEF1-HTLV prom
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    105852-plasmid-map-sequence-id-206014

    Enlarge

  29. Adeno BN

    Plasmid
    #101823
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1
    Depositor
    Andrea Ventura
    Article
    Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10
    Insert
    U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)_CBh_FLAG-Cas9 (Bcan, Ntrk1 Mouse)
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Expression
    Mutation
    Promoter
    Available since
    Nov. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  30. pMCh-1538

    Plasmid
    #82431
    Purpose
    Plasmid for expression of NHA-tagged human-Nematostella GW182 11W11A chimera in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    hs-nvGW182 11W11A chimera
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …
    Promoter
    CMV
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82431-plasmid-map-sequence-id-156186

    Enlarge

  31. Progerin-S22D-CS-pLPC

    Plasmid
    #73810
    Purpose
    Progerin mutant where Serine 22 is mutated to aspartic acid and where there is a substitution of cysteine in CaaX-motif by a serine
    Depositor
    Gerardo Ferbeyre
    Article
    Moiseeva et al Aging (Albany NY). 2016 Feb;8(2):366-81.
    Insert
    Progerin (LMNA Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Progerin with substitution of cysteine in CaaX-mo…
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  32. pLPC-S22AProgerin-CS

    Plasmid
    #69063
    Purpose
    encodes a Progerin mutant with serine 22 mutated to an alanine and with a substitution of cysteine in CaaX-motif by a serine
    Depositor
    Gerardo Ferbeyre
    Article
    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d
    Insert
    Progerin (LMNA Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Progerin with serine 22 mutated to alanine and wi…
    Promoter
    CMV
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  37. CA-RIT-NFAT1 (IRES-GFP)

    Plasmid
    #85181
    Purpose
    Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selection
    Depositor
    Anjana Rao
    Article
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    CA-RIT-NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Amino acids 1-3 removed; CA: PASSGSSASF mutated t…
    Promoter
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  38. kiCAP-AAV-MyoAAV2A

    Plasmid
    #224440
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDQTTL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    224440-plasmid-map-sequence-id-446665

    Enlarge

  39. Ma-pREP

    Plasmid
    #197571
    Purpose
    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    111TAG and 8TAG/112TAG
    Promoter
    AraC, T7, cat promoter (constitutive), and lpp (c…
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197571-plasmid-map-sequence-id-389424

    Enlarge

  40. pCMV-PE6b

    Plasmid
    #207852
    Purpose
    Mammalian expression of PE6b prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6b
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207852-plasmid-map-sequence-id-408170

    Enlarge

  41. kiCAP-AAV-MyoAAV4A

    Plasmid
    #224448
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYNSL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    224448-plasmid-map-sequence-id-446642

    Enlarge

  43. kiCAP-AAV-MyoAAV1A

    Plasmid
    #224437
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 1A, a muscle-tropic AAV capsid in mice.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDLTTP insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    224437-plasmid-map-sequence-id-446668

    Enlarge

  44. kiCAP-AAV-MyoAAV4E

    Plasmid
    #224452
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDFNNT insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    224452-plasmid-map-sequence-id-446621

    Enlarge

  46. pFUSE2ss-CLIg-mK_M18

    Plasmid
    #82358
    Purpose
    Expression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103
    Insert
    mouse immunoglobulin kappa light chain, variable fragment derived from clone M18 (Igk, Igkc, Igk-V Mouse)
    Use
    Tags
    none
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1-HTLV prom
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    82358-plasmid-map-sequence-id-400982

    Enlarge

  49. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid
    #216695
    Purpose
    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)
    Depositor
    Franck Perez
    Article
    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s
    Inserts
    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    Use
    Tags
    FIREtag, Streptavidin Binding Protein (SBP), and …
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216695-plasmid-map-sequence-id-429706

    Enlarge

  50. kiCAP-AAV-MyoAAV1E

    Plasmid
    #224439
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 1E, a muscle-tropic AAV capsid in mice.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDTMSK insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224439-plasmid-map-sequence-id-446666

    Enlarge

Showing: 5301 - 5350 of 9339 results