We narrowed to 13,723 results for: Cas9

1. pLenti Vp64-dCas9 T2A mTagBFP2

   Plasmid

   #171052

   Purpose

   Expression of Vp64-dCas9 activation system

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   Vp64-dCas9 T2A mTagBFP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

   Plasmid

   #60903

   Purpose

   Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   Cas9 dead
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-EF1a-dCas9-VPR-Puro

   Plasmid

   #99373

   Purpose

   3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   (Sp)dCas9-VPR-P2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRH-1179 U6-reci Gag-Cas9 v2

   Plasmid

   #201914

   Purpose

   Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Gag-Cas9 v2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

   Plasmid

   #159914

   Purpose

   Mutagenesis of Rosa26 with SauCas9

   Depositor

   Larry Zweifel

   Insert

   Rosa26 gRNA (Gt(ROSA)26Sor Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-FLEXfrt-SaCas9-U6-sgRosa26

   Plasmid

   #159915

   Purpose

   Mutagenesis of Rosa26

   Depositor

   Larry Zweifel

   Insert

   Rosa26 (Gt(ROSA)26Sor Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-CFP-SaCas9-HF (without sgRNA)

   Plasmid

   #134470

   Purpose

   Human expression vector for SaCas9-HF variant.

   Depositor

   Zongli Zheng

   Article

   Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

   Insert

   Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
   

   Use

   CRISPR

   Tags

   SV40NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   R245A, N413A, N419A, R654A

   Promoter

   CAG

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

   Plasmid

   #209196

   Purpose

   Mutagenesis of Cnr1

   Depositor

   Larry Zweifel

   Article

   Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

   Insert

   Cnr1 (Cnr1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-dCas9-p300-P2A-PuroR

    Plasmid

    #83889

    Purpose

    Lentiviral Sp dCas9-p300-P2A-PuroR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    pac from Streptomyces alboniger
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid

    #106964

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-EF1a-dCas9-KRAB-Puro

    Plasmid

    #99372

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-KRAB-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-UbC-VP64-dCas9-VP64

    Plasmid

    #232179

    Purpose

    Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    VP64-dCas9-VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

    Plasmid

    #221550

    Purpose

    Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human TBK1 (TBK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP

    Plasmid

    #234881

    Purpose

    L1HS-silencing plasmid (CRISPRi gRNA2)

    Depositor

    Johan Jakobsson

    Article

    Adami et al bioRxiv 2025.01.17.633315

    Insert

    LacZ gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

    Plasmid

    #234882

    Purpose

    L1HS-silencing plasmid (CRISPRi gRNA1)

    Depositor

    Johan Jakobsson

    Article

    Adami et al bioRxiv 2025.01.17.633315

    Insert

    L1HS-silencing plasmid (CRISPRi gRNA1)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

    Plasmid

    #154139

    Purpose

    Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
    

    Use

    Tags

    3x FLAG and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pE-35S-miniSdd7-nCas9-PBE-GmPPO2

    Plasmid

    #204855

    Purpose

    For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI-mScarlet
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    35S, AtU6-26p

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-LTR-dCas9-VP64-BFP

    Plasmid

    #46912

    Purpose

    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    dCas9-VP64-BFP fusion
    Puromycin resistance
    

    Use

    CRISPR and Retroviral

    Tags

    3xNLS, BFP, and VP64 domain

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and PGK

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

    Plasmid

    #180263

    Purpose

    Lentiviral expression vector for dCas9-VP64 in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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