Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 14,223 results for: Cas9

Clear keywords
Showing: 2121 - 2140 of 14223 results

  5. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid
    #100597
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)
    Depositor
    Akitsu Hotta
    Publication
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100597-plasmid-map-sequence-id-207349

    Enlarge

  8. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

    Plasmid
    #216733
    Purpose
    Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.
    Depositor
    Vincent Dion
    Publication
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase and sgCTG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    U6 and CAG
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216733-plasmid-map-sequence-id-430318

    Enlarge

  12. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid
    #60600
    Purpose
    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.
    Depositor
    Akitsu Hotta
    Publication
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CRISPR Cas9 D10A
    puromycin resistance gene
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Changed Asp 10 to Ala, Codon usage optimized for …
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60600-plasmid-map-sequence-id-97110

    Enlarge

  13. HeFm2SpCas9

    Plasmid
    #92111
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    “Highly enhanced Fidelity” mut2 SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92111-plasmid-map-sequence-id-197616

    Enlarge

  14. pLCHKO_GFP_Luciferase

    Plasmid
    #155079
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155079-plasmid-map-sequence-id-308671

    Enlarge

  15. pLCHKO_intergenic-intergenic_2

    Plasmid
    #155078
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155078-plasmid-map-sequence-id-308534

    Enlarge

  18. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid
    #196722
    Purpose
    Tet-inducible expression of Cas9-T2A-miRFP670nano
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196722-plasmid-map-sequence-id-389804

    Enlarge

  19. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139094
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139094-plasmid-map-sequence-id-273891

    Enlarge

Showing: 2121 - 2140 of 14223 results