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pWJPrP34
Plasmid#22107DepositorInsertPrP exon 3 EcoR1 fragment
UseConstruction of targeting vectorTagsExpressionMutationAdded a Cla1 site right before translation start …PromoterAvailable sinceOct. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMetYC-Dest
Plasmid#105081PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.DepositorTypeEmpty backboneUseTagsCub-PLVExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
POM43-mTFP1
Plasmid#69853PurposeN-terminal tagging with mTFP1 using LEU2 as a selection marker in S. cerevisiae.DepositorInsertmTFP1 minus start and stop codon
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
RSRF-Luc-2wt
Plasmid#31818DepositorInsertMEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
UseLuciferaseTagsExpressionMammalianMutationPromoterMEF2-dependent (-307 to -242 of Nur77/NR4A1)Available sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSCW01
Plasmid#72300PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19TagsExpressionBacterialMutationPromoterlacAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSCW02
Plasmid#72301PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19TagsExpressionBacterialMutationPromoterlacAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH136
Plasmid#47554PurposepJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.DepositorInsertURA3
UsePcr templateTagsExpressionMutationPromoterNative URA3 promoter, -243 to -1.Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pRS263
Plasmid#122379PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS267
Plasmid#122380PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_full-site
Plasmid#186277PurposeReporter plasmid for full-site selection assayDepositorInsertThiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-Rabaptin-5
Plasmid#199173PurposeExpresses GFP-Rabaptin-5 fusion in mammalian cellsDepositorInsertRabaptin-5 (RABEP1 Human)
UseTagsEGFPExpressionMammalianMutationContains Tyr 149 Ser substitution; insert starts …PromoterCMVAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4137386
Plasmid#149540PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Human)
UseTagsExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmIL-6 -231
Plasmid#61287Purposedrives luciferase via mouse IL-6 promoter, truncated (-231)DepositorInsertTruncated IL-6 promoter
UseLuciferaseTagsExpressionMammalianMutationDeletion of 1046 nucleotides from the 5' end…PromoterIL-6 promoter (truncated)Available sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 hNLRP3-mNG-STOP
Plasmid#218644PurposeGateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)DepositorInsertNLRP3 (NLRP3 Human)
UseGateway entryTagsmNeonGreenExpressionMutationPromoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-RNF40i1
Plasmid#59594PurposeExpression of shRNA against human RNF40DepositorInsertRNF40 (RNF40 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla/PAG1-TurboID-GAI
Plasmid#198217PurposeTransposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labelingDepositorInsertPAG1 (PAG1 Human)
UseTransposonTags3’ part of TurboID, starting with G257 and amino …ExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
DLC1A
Plasmid#25333PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDLC1:S1074-L1283 (DLC1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids S1074-L1283PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSicoR-RNF40i2
Plasmid#59595PurposeExpression of shRNA against human RNF40DepositorInsertRNF40 (RNF40 Human)
UseRNAiTagsExpressionMammalianMutationPromoteruAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only
