We narrowed to 28,582 results for: ple

1. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

   Plasmid

   #109428

   Purpose

   A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   mCherry T2A GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Inserted 43 base pairs into mCherry to create rep…

   Promoter

   CMV

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2

   Plasmid

   #178574

   Purpose

   Lentiviral vector for CamKIIα::Opto-vTrap

   Depositor

   Won Do Heo

   Article

   Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

   Insert

   CRY2PHR-P2A-CIBN-mCherry-VAMP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2

   Plasmid

   #178575

   Purpose

   Lentiviral vector for GFAP::Opto-vTrap

   Depositor

   Won Do Heo

   Article

   Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

   Insert

   CRY2PHR-P2A-CIBN-mCherry-VAMP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   GFAP

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. plenti-CAG-IKZF1-V1-Q146H-FLAG-IRES-GFP

   Plasmid

   #69048

   Purpose

   lentiviral expression of IKZF1 variant 1 with Q146H mutation and FLAG tag

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF1 (IKZF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Mutation

   splice variant 1, Q146H

   Promoter

   CAG

   Available since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47869

   Purpose

   All-in-one plasmid targeting tdTomato

   Depositor

   James Thomson, Erik Sontheimer

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Inserts

   NmCas9
   U6pr-tracrRNA
   tdTomato crRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGB3_alpha2: Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos-Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1679)

   Plasmid

   #160619

   Purpose

   Module for estradiol-inducible expression of the PhiC31 integrase gene and dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   ERLexABDGal4AD / PhiC31 / GRLacIBDGal4AD / RDF
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

   Plasmid

   #215014

   Purpose

   Express an autophagy (LC3) reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   MAP1LC3B (Map1lc3b Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti HsATP10B_WT-T2A-His-flag-TMEM30A variant1

   Plasmid

   #191675

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A

   Depositor

   Veerle Baekelandt

   Inserts

   ATP10B (ATP10B Human)
   TMEM30A (TMEM30A Human)
   

   Use

   Lentiviral

   Tags

   6X his and flag

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-Zyxin-Full Length (aa1-564)-EGFP

   Plasmid

   #187692

   Purpose

   Visualization of Zyxin

   Depositor

   Mary Beckerle

   Article

   Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

   Insert

   Zyxin (Zyx Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX304-GFP-IQSEC1 v2-E620K GEF dead mutant

    Plasmid

    #162020

    Purpose

    Expression of GEF dead IQSEC1 mutant

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EK

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro

    Plasmid

    #120560

    Purpose

    Expresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    HRAS (HRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CMV-SLC7A11-sh926R-FLAG-IRES-Hygro

    Plasmid

    #118702

    Purpose

    lentiviral vector experessing SLC7A11 insensitive to hairpin TRCN0000288926

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    SLC7A11 (SLC7A11 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    wt , SLC7A11 sh926R

    Promoter

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47870

    Purpose

    All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

    Depositor

    James Thomson, Erik Sontheimer

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Inserts

    NmCas9
    U6pr-tracrRNA
    OCT4 crRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX-uORF-HA-birA*-H-Ras(wildtype)-IRES-Puro

    Plasmid

    #120559

    Purpose

    Expresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    HRAS (HRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

    Plasmid

    #120564

    Purpose

    Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

    Plasmid

    #163644

    Purpose

    Makes a lentivirus that expresses mNeonGreen and Cas9.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    mNeonGreen-P2A-Cas9
    

    Use

    Lentiviral

    Tags

    mNeonGreen-P2A

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro

    Plasmid

    #177201

    Purpose

    encoding a dominant negative form of CEBP under inducible promoter

    Depositor

    Markus Covert

    Article

    Kudo et al bioRxiv (2022)

    Insert

    dnCEBP (A-C/EBP)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

    Plasmid

    #120563

    Purpose

    Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]

    Plasmid

    #159802

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc

    Plasmid

    #138368

    Purpose

    Lentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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