162,981 results

1. HA-HA-AR-DBD-LBD

   Plasmid

   #171237

   Purpose

   Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   AR truncate

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TB:6xAP-1_RE:MiniP

   Plasmid

   #133884

   Purpose

   6 copies of the AP-1 binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   6 copies of the AP-1 binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKO403_lacZ_Sp

   Plasmid

   #174726

   Purpose

   Bifidobacterium-E.coli shuttle vector for seamless gene cloning

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS L30 mRuby3-Rab27A

   Plasmid

   #166432

   Purpose

   Rab27A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

   Depositor

   Raphael Gaudin

   Insert

   Rab27A
   

   Use

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   L30 weak promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUGW-APOE-E4-T2A-mCherry

   Plasmid

   #190809

   Purpose

   Express APOE E4 allele from Ubiquitin C promoter, co-expressing mCherry

   Depositor

   Ronald Hart

   Insert

   APOE (APOE Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   E4

   Promoter

   Ubi C

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-GCaMP6fu

   Plasmid

   #86235

   Purpose

   Express fluorescent calcium indicator GCaMP6fu in mammalian cells

   Depositor

   Katalin Torok

   Article

   Helassa et al Sci Rep. 2016 Dec 6;6:38276. doi: 10.103

   Insert

   GCaMP6fu
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W43Y D395A

   Promoter

   CMV

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLC-254

   Plasmid

   #124291

   Purpose

   Expression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.

   Depositor

   Swaine Chen

   Article

   Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4

   Insert

   vsfGFP-0
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

   Plasmid

   #107943

   Purpose

   Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
   

   Use

   Tags

   3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A

   Promoter

   CAG

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBiFC-bFosVC155

   Plasmid

   #22013

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   bFosVC155
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Oct. 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3_cAMPFIRE-L

    Plasmid

    #182279

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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