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Plasmid#189799PurposeRetroviral delivery of control guide RNADepositorInsertLuciferase gRNA
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_LAMP1
Plasmid#65685PurposeExpresses LAMP1-CNL in mammalian cellsDepositorInsertLAMP1
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_LAMP1
Plasmid#65684PurposeExpresses LAMP1-YNL in mammalian cellsDepositorInsertLAMP1
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMV306hsp+LuxG13
Plasmid#26161DepositorInsertBacterial luciferase operon + G13 promoter
UseTagsExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3:Luc
Plasmid#192986PurposePlasmid to test the Hydra Wnt3 promoter activityDepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad-LgBiT-PhoCl1-SmBiT-MBP
Plasmid#164034Purposeluciferase-based PhoCl screening constructDepositorInsertLgBiT-PhoCl1-SmBiT-MBP
UseTagsHis tag, T7 tag, Xpress TagExpressionBacterialMutationPromoteraraBad promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHLuc Control
Plasmid#141091PurposepH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEPDepositorInsertAntares-T2A-puromycin-IRESv24-eGFP-Nanoluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR
Plasmid#160042PurposeFor the auxin receiver that report on auxin.DepositorInsertsosTIR1
BlastR
UseLuciferaseTagsmAID and mCherryExpressionMammalianMutationPromoterAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PTEN 3'UTR
Plasmid#28104DepositorInsertPTEN 3'UTR (Pten Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Nluc-3xP3-RFP
Plasmid#127563PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.DepositorInsertNluc
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-Polr2a promoter_600bps
Plasmid#21111DepositorInsertPolr2a promoter_600bps (POLR2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTH646-2µ-RLuc
Plasmid#40600DepositorInsertRenilla Luciferase
UseTagsExpressionYeastMutationPromoterADH1Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc-IRES-Antares
Plasmid#141085Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEPLuc-EMCV IRES-Antares
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc
Plasmid#141086Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertAntares-T2A-puromycin-EMCV IRES-SEPLuc
UseTagsExpressionMammalianMutationDeletion of amino acids 66 to 91 on the Luciferas…PromoterCMVAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoA-91-NLuc
Plasmid#207151PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)GPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoB-91-NLuc
Plasmid#207152PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)GPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
HySp5:Luc
Plasmid#192985PurposePlasmid to test the Hydra Sp5 promoter activityDepositorInsertHydra Sp5 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D5:Luc
Plasmid#193007PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D5)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D5PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D4:Luc
Plasmid#193006PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D4)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D4PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D6:Luc
Plasmid#193008PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D6)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D6PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D1:Luc
Plasmid#193003PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D1)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D1PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D3:Luc
Plasmid#193005PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D3)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D3PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D2:Luc
Plasmid#193004PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D2)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D2PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZfWnt3:Luc
Plasmid#192992PurposePlasmid to test the Zebrafish Wnt3 promoter activityDepositorInsertZebrafish Wnt3 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc IRESv24
Plasmid#141087Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRESDepositorInsertAntares-T2A-puromycin-IRESv24-SEPLuc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH729-minRLuc
Plasmid#38225DepositorInsertRenilla Luciferase
UseTagsExpressionYeastMutationEvery codon of the Renilla coding sequence was ex…PromoterTDH3 (=GPD)Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSLGCV
Plasmid#49862PurposeExpression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).DepositorInsertfirefly luciferase luc+ fused to GFP (S65C)
UseTagsGFP (S65C)ExpressionWormMutationPromotersur-5Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-NLuc_SV40:PuroR
Plasmid#183045PurposeExpresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.DepositorInsertNanoLuciferase
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTetON-EGFP
Plasmid#89453PurposeTet/Dox-inducible expression of EGFP in mammalian cells (control)DepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
FL-mCherry
Plasmid#78862PurposeDULIP negative control (prey) to exclude unspecific background bindingDepositorInsertmCherry
UseExpression vectorTagsFirefly luciferase, V5-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only