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We narrowed to 18,759 results for: nls

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Showing: 4281 - 4320 of 18759 results

  3. ARB366

    Plasmid
    #124049
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124049-plasmid-map-sequence-id-252514

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  4. ARB367

    Plasmid
    #124050
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124050-plasmid-map-sequence-id-252515

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  6. pMIIS928

    Plasmid
    #238211
    Purpose
    Expresses TaTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.
    Depositor
    Feng Zhang
    Publication
    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TaTasR
    Tags
    HA-NLS
    Expression
    Mammalian
    Available since
    May 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    238211-plasmid-map-sequence-id-473286

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  7. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

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  11. pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

    Plasmid
    #139990
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VQR=D1135V/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139990-plasmid-map-sequence-id-275521

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  13. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

    Plasmid
    #139992
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139992-plasmid-map-sequence-id-275524

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  14. pCMV-bpNLS-Stab-exin21-Bxb1(A315R)-Stab (CJT605)

    Plasmid
    #248211
    Purpose
    bpNLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586
    Insert
    bpNLS-Stab-exin21-Bxb1(A315R)-Stab
    Use
    CRISPR; In vitro transcription
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Bxb1(A315R)
    Promoter
    CMV and T7
    Available since
    April 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248211-plasmid-map-sequence-id-506607

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  16. pPBneo-CIBN-lin-p53CT(98-393)-6KQ-mNeonGreen-NLSx3

    Plasmid
    #241848
    Purpose
    Expresses an improved localizer of Opto-p53 in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Publication
    Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p53 (TP53 Human)
    Tags
    CIBN and mNeonGreen
    Expression
    Mammalian
    Mutation
    p53 C-terminus (97-393 aa) containing six acetyla…
    Promoter
    CAGGS promoter
    Available since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    241848-plasmid-map-sequence-id-485119

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  17. pPBbsr2-NLSx3-miRFP703-dSal-p53NT(1-97)-5SD-lin-hCRY2

    Plasmid
    #241846
    Purpose
    Expresses an improved actuator of Opto-p53 in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Publication
    Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p53 (TP53 Human)
    Tags
    CRY2 and miRFP703
    Expression
    Mammalian
    Mutation
    N-terminus (1-97 aa) containing five phosphorylat…
    Promoter
    CAGGS promoter
    Available since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    241846-plasmid-map-sequence-id-485117

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  32. pSmart-EF1a-Nla-cas5

    Plasmid
    #178878
    Purpose
    Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas5c-bGH polyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178878-plasmid-map-sequence-id-351751

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  33. pSmart-EF1a-Nla-cas7

    Plasmid
    #178879
    Purpose
    Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas7c-bGH PolyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178879-plasmid-map-sequence-id-351739

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  34. pSmart-EF1a-Nla-cas8

    Plasmid
    #178880
    Purpose
    Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas8c-bGH PolyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178880-plasmid-map-sequence-id-351750

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  35. pSmart-EF1a-Nla-cas11

    Plasmid
    #178882
    Purpose
    Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas11c-bGH polyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178882-plasmid-map-sequence-id-351753

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  36. MSP2279

    Plasmid
    #70706
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A and H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70706-plasmid-map-sequence-id-127768

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  37. MSP2266

    Plasmid
    #70705
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70705-plasmid-map-sequence-id-131909

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  39. mKikGR-PCNA-19-SV40NLS-4

    Plasmid
    #57328
    Purpose
    Localization: Nucleus, Excitation: 507 / 583, Emission: 517 / 593
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mKikGR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. SpCas9-ABE8e(V106W)

    Plasmid
    #198547
    Purpose
    Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198547-plasmid-map-sequence-id-388882

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Showing: 4281 - 4320 of 18759 results