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Plasmid#190079Purposeexpress chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance geneDepositorInsertACE2 (TMPRSS2 Synthetic, Rhinolophus alcyone and Rhinolophus ferrumequinum)
UseTagsExpressionMammalianMutationPromoternoneAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine
Plasmid#186419PurposeExpression of shRNA for the knock down of mouse Egr3DepositorInsertmiREgr3-5D
UseAAVTagsmCitrineExpressionMutationPromoterhuman Synapsin1Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)
Plasmid#185717PurposeAAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoterDepositorInsertsynuclein, alpha (SNCA Human)
UseAAVTagsExpressionMutationChanged Ala 11 to Pro, Val 70 to ProPromoterhSynAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-nCoV2019-N-YBBR-2xStrep-IRES-Puro
Plasmid#185450PurposeExpresses SARS-CoV-2 nucleocapsid protein with a c-terminal YBBR and Strep tag in mammalian cellsDepositorInsertNucleocapsid (N SARS-CoV-2)
UseLentiviralTagsStrep-Tag II and YBBRExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1
Plasmid#183399PurposeDual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-NanoLuc-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-redesigned-human-Caspase-4_ANR261ENP_D315H_M318K_T352K
Plasmid#183357PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4 with ICE activity (CASP4 Human)
UseRetroviralTagsMycExpressionMutationANR261ENP, D315H, M318K, T352KPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro
Plasmid#166134PurposeThis plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistanceDepositorInserthU6-gRNA and hH1-gRNA targeting firefly luciferase gene
UseCRISPR and LentiviralTagsiRFP670ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP1069-Ai210 (TITL-GC7f-ICF-IRES-tTA2)
Plasmid#181763Purposetargeting vectorDepositorInsertGCaMP7f and tTA2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP993-Ai195 (TIT2L-GC7s-ICF-IRES-tTA2)
Plasmid#181762Purposetargeting vectorDepositorInsertGCaMP7s and tTA2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND1(T286I)-IRES-mCherry
Plasmid#172638PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286I) and mCherry in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationThr286IlePromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND1(P287L)-IRES-mCherry
Plasmid#172636PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287L) and mCherry in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationPro287LeuPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(L301R/V303R)-IRES-neo
Plasmid#128664Purposemammalian expression of Flag-Polß(L301R/V303R) protein with neomycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303RPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB6-Id1-IRES-loxP-NeoR-loxP-DTA-TK
Plasmid#158616PurposeB6J Id1-LSL-DTA allele targeting vector, low copy numberDepositorInsertId1 (Id1 Mouse)
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationInsertion of IRES-loxP-NeoR-loxP-DTA after the st…PromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(L301R/V303R)-IRES-puro
Plasmid#128658Purposemammalian expression of Flag-Polß(L301R/V303R) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303RPromoterCMVAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(L301R/V306R)-IRES-puro
Plasmid#128656Purposemammalian expression of Flag-Polß(L301R/V306R) with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V306RPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(K206A/K244A)-IRES-puro
Plasmid#128683Purposemammalian expression of Flag-Polß(K206A/K244A) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationK206A/K244APromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(TM/K206A/K244A)-IRES-puro
Plasmid#128684Purposemammalian expression of Flag-Polß(TM/K206A/K244A: L301R/V303R/V306R/K206A/K244A)) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlag tagExpressionMammalianMutationL301R/V303R/V303R/K206A/K244APromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-392 HA Venus173/155[1] IRES Venus173/155[2]
Plasmid#130140PurposePPI (BiFC/TriFC) assay vectorDepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B V304A,I367A IRES GFP
Plasmid#133821PurposeEncodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationV304A, I367APromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A K189-192A IRES GFP
Plasmid#133823PurposeEncodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationK189-192APromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only