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LT2_32_PyruEth_X2site

Plasmid

#194428

Purpose

Plasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

PyruEth

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_75_Donor_Tag_RPL13A-mTurq2

Plasmid

#194415

Purpose

Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

mTurquoise2 CDS

Use

Synthetic Biology; Used as donor dna in genome in…

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLY227

Plasmid

#184148

Purpose

short crRNA generator with spacer LEA2 with WT repeat region

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-crRNA-LEA2-WT

Use

Synthetic Biology

Promoter

Plux2

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Hygro-Bem1-mCherry-AID

Plasmid

#173925

Purpose

Plasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AID

Depositor

Liedwij Laan

Article

Daalman et al Philos Trans R Soc Lond B Biol Sci. 2023

Inserts

Partial Bem1 C-terminus
hygromycin marker
Bem1 downstream sequence

Tags

mCherry- AID

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLY171

Plasmid

#184145

Purpose

sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

sgRNA-LEA2-ex3

Use

Synthetic Biology

Promoter

Plux2

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP RanSen

Plasmid

#184697

Purpose

Lentiviral transfer plasmid for expression of GFP RanSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP RanSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-ArgSen (-)NLS

Plasmid

#184689

Purpose

Lentiviral transfer plasmid for expression of ArgSen (-)NLS

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen (-)NLS

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-ArgSen

Plasmid

#184691

Purpose

Lentiviral transfer plasmid for expression of ArgSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-ArgSen

Plasmid

#184700

Purpose

Lentiviral transfer plasmid for expression of ArgSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-tdTomato-WPRE

Plasmid

#187112

Purpose

FLP-dependent expression of tdTomato under EF1a promoter

Depositor

Yongling Zhu

Article

Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

Insert

tdTomato

Use

AAV

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2370-Tier1-PhCMV-TetR_F-type

Plasmid

#169573

Purpose

Tier-1 vector encoding PhCMV-driven tetracycline-dependent F-type-based transactiavtor (PhCMV-TetR-F-type-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled transactivator

Expression

Mammalian

Promoter

PhCMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM262 - pDESTR4-R3 mosTI hygroR target

Plasmid

#159840

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI hygroR target

Expression

Worm

Mutation

Not applicable

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-9-35S-11

Plasmid

#176881

Purpose

MoClo Level 0 plasmid, contains 35S

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

35S terminator

Expression

Plant

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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gSCPL35-mRFP

Plasmid

#181960

Purpose

SCPL35 protein tagged with mRFP, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

SCPL35

Tags

mRFP

Expression

Plant

Promoter

native SCPL35

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-tdMCP2-GyG-(GGS)X6-HaloTag C261

Plasmid

#171434

Purpose

Expression of tdMCP2-GyG-(GGS)X6-HaloTag C261 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

tdMCP2-GyG-(GGS)X6-HaloTag C261

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2365 - Intron GG1 - 250bp no PATC

Plasmid

#159883

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - 250bp no PATC

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2345 - Intron GG2 - 250bp no PATC

Plasmid

#159884

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 250bp no PATC

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2366 - intron GG3 - 250bp no PATC

Plasmid

#159885

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp no PATC

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV RhoB YFP MS2

Plasmid

#131777

Purpose

RhoB upstream msfYFP 24xMS2 RhoB downstream for CRISPR tagging

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

msfYFP - 24x MS2 loops

Use

CRISPR

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV Btg2 YFP MS2

Plasmid

#131776

Purpose

Btg2 upstream msfYFP 24xMS2 Btg2 downstream for CRISPR tagging

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

msfYFP - 24x MS2 loops

Use

CRISPR

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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