We narrowed to 148,810 results for: ins;

1. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

   Plasmid

   #185918

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFX-iRFP-Rab5

   Plasmid

   #174456

   Purpose

   Express iRFP with early endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   iRFP with early endosome-targeting sequence
   

   Tags

   small GTPase (Rab5)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJAK184.tetR.PT5-3.dCas9

   Plasmid

   #232482

   Purpose

   optimized ahTet inducible dCas9 for insertion into C. difficile genome

   Depositor

   James Collins

   Article

   Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

   Insert

   Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   TetR-PT5-3

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJC.15A.sgRNA.TA

   Plasmid

   #232483

   Purpose

   sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

   Depositor

   James Collins

   Article

   Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

   Inserts

   Toxin-antitoxin System from CD630
   medium-copy-number p15A origin of replication
   mCherry
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   P3 constitutive promoter and native promoter

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330_DRS-1 sgRNA / hSpCas9

   Plasmid

   #172841

   Purpose

   Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFX-β-tubulin-mCherry

   Plasmid

   #175829

   Purpose

   Express mCherry with microtubule-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with microtuble-targeting sequence
   

   Tags

   human tubulin beta 4B class IVb;TUBB4B

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-Amp_CRISPIE donor B1

   Plasmid

   #172842

   Purpose

   CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mEGFP
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI_AFF-1-FLAG_H2B-RFP

   Plasmid

   #240506

   Purpose

   Cell fusion assays

   Depositor

   Benjamin Podbilewicz

   Article

   Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

   Insert

   AFF-1
   

   Tags

   H2B-RFP

   Expression

   Mammalian

   Available Since

   July 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-nef-CIAO2-TVA-mCherry

   Plasmid

   #149297

   Purpose

   Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

   Depositor

   Edward Callaway

   Article

   Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

   Insert

   TVA:mCherry
   

   Use

   AAV

   Mutation

   Start codon removed.

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFX-EGFP-Rab7

    Plasmid

    #174457

    Purpose

    Express EGFP with late endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with late endosome-targeting sequence
    

    Tags

    small GTPase (Rab7)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMW41: pET-His-MBP-SUMO-R2Bm-SII

    Plasmid

    #209290

    Purpose

    Expresses the R2Bm ORF in E. coli

    Depositor

    Feng Zhang

    Article

    Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

    Insert

    R2
    

    Tags

    His, MBP, StrepII, and bdSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFX-EGFP-Rab5

    Plasmid

    #174454

    Purpose

    Express EGFP with early endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with early endosome-targeting sequence
    

    Tags

    small GTPase (Rab5)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFX-iRFP-Rab7

    Plasmid

    #174459

    Purpose

    Express iRFP with late endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with late endosome-targeting sequence
    

    Tags

    small GTPase (Rab7)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pREDIT_MCP-RecT

    Plasmid

    #164803

    Purpose

    mammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Insert

    RecT
    

    Expression

    Mammalian

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFX-β-tubulin-EGFP

    Plasmid

    #175827

    Purpose

    Express EGFP with microtubule-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with microtuble-targeting sequence
    

    Tags

    human tubulin beta 4B class IVb;TUBB4B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOCC305

    Plasmid

    #118898

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and TwinStrep-MB…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-FKBP-SYNDAPIN2

    Plasmid

    #221311

    Purpose

    Expression of SYNDAPIN2 (pLxIS) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    SYNDAPIN2 (pLxIS) (Pacsin2 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG-4xFKBP

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. RIP Cre-ER (DM#265)

    Plasmid

    #15029

    Depositor

    Douglas Melton

    Article

    Dor et al Nature. 2004 May 6. 429(6987):41-6.

    Insert

    Rat insulin promoter (RIP) (Ins2 Rat)
    

    Use

    Cre/Lox; For making transgenic constructs

    Tags

    Cre-ER (TM)

    Expression

    Mammalian

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTriEx-mCherry::LANS4

    Plasmid

    #60785

    Purpose

    Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

    Insert

    LANS4
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFX-mCherry-KRasCT

    Plasmid

    #175835

    Purpose

    Express mCherry with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with plasma membrane-targeting sequence
    

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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