We narrowed to 14,579 results for: pET

1. pET21-pelB-LaG-8

   Plasmid

   #172761

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-8
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-5

   Plasmid

   #172760

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-5
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FKBP-YPet-RanGAP*

   Plasmid

   #175245

   Purpose

   Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   RANGAP1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Amino acid 562 F to A

   Promoter

   tac

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET30a BCP1 NTase

   Plasmid

   #195245

   Purpose

   Expresses phage BCP1 NTase

   Depositor

   Douglas Feldman

   Article

   Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

   Insert

   BCP1 phage NTase
   

   Use

   Tags

   His6 and SBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30a-Av05 NTase

   Plasmid

   #195243

   Purpose

   Expresses phage Av05 NTase

   Depositor

   Douglas Feldman

   Article

   Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

   Insert

   phage Av05 NTase
   

   Use

   Tags

   His6 and SBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-BCA-Pep-H

   Plasmid

   #185782

   Purpose

   Encodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   BCA-Pep-H
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB1C3_YPet-PCP (y-plasmid)

   Plasmid

   #127637

   Purpose

   Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

   Insert

   YPet
   

   Use

   Synthetic Biology

   Tags

   RNA binding peptide: PP7 coat protein (PCP)

   Expression

   Bacterial

   Mutation

   Promoter

   pJ21306

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_6xHis-PARP1-A774L

   Plasmid

   #174796

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A774L (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A774L, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_6xHis-PARP1-W318R

   Plasmid

   #174793

   Purpose

   Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-W318R (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   W318, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. D5 gene in PETCON vector

    Plasmid

    #87626

    Purpose

    expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

    Insert

    Designed allosteric Zn2+ and fluorescein binding antibody scFv D5
    

    Use

    Tags

    3x(G4S) Linker and cMyc tag on backbone

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET_RGG-RFP-RGG

    Plasmid

    #124944

    Purpose

    Exoression of RGG-RFP-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET151-mUCH37 (ISF2)

    Plasmid

    #61928

    Purpose

    Expression of mUCH37 (ISF2) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    UCH37 (M.musculus) (Uchl5 Mouse)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBC2T-Ypet-FtnA

    Plasmid

    #108969

    Purpose

    expresses BC2-tag-Ypet-FtnA in mammalian cells

    Depositor

    Ulrich Rothbauer

    Article

    Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

    Insert

    FtnA (ftnA Escherichia coli)
    

    Use

    Tags

    BC2-tag and Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET30Xa-SBL1(160S)

    Plasmid

    #66872

    Purpose

    Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)
    

    Use

    Tags

    Histag from pET30Xa-LIC

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7lac

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM-30-GST-Dmp68_B

    Plasmid

    #145962

    Purpose

    Bacterial Expression of Dmp68

    Depositor

    Elisa Izaurralde

    Insert

    Dmp68 (Rm62 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21b - S. thermophilus DltB

    Plasmid

    #132607

    Purpose

    expresses S. thermophilus DltB in E. coli

    Depositor

    Wenqing Xu

    Article

    Ma et al Nature. 2018 Oct;562(7726):286-290. doi:

    Insert

    Dltb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-mAIM2-GST

    Plasmid

    #51533

    Purpose

    Express GST-tagged mAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    Aim2 (Aim2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAR69_STRI_m(KS_MAT)_H8_pET22b

    Plasmid

    #122849

    Purpose

    Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    m(KS_MAT) (Fasn Mouse)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET15b-His-IkappaBalpha(FL)

    Plasmid

    #44738

    Depositor

    Gourisankar Ghosh

    Insert

    IkappaBalpga (Nfkbia Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pETM6-H10-mCherry

    Plasmid

    #66531

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter H10

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - H10

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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