We narrowed to 8,683 results for: sgRNA

1. pENTR-AmCyan

   Plasmid

   #138481

   Purpose

   For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   AmCyan
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PL-5LTR-RGR(DMD​#​1​)-AmCyan-A

   Plasmid

   #138482

   Purpose

   Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Ribozyme-flanked gRNA and AmCyan
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDYT001

   Plasmid

   #186549

   Purpose

   Lineage tracing vector (with barcoded Target Sites and triple sgRNAs)

   Depositor

   Jonathan Weissman

   Article

   Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00

   Insert

   14-bp random integration barcode and three target sites and 3x sgRNAs
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PL-5LTR-GW-A

   Plasmid

   #138480

   Purpose

   For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-U6-sgMettl3

   Plasmid

   #196263

   Purpose

   Expresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoter

   Depositor

   Laura Attardi

   Article

   Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

   Insert

   Mettl3 sgRNA (Mettl3 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. peSCBE3-NG-HF1-Hypa

   Plasmid

   #218166

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-HF1-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-AAVS1

   Plasmid

   #227272

   Purpose

   Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting AAVS1 (AAVS1 Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGRACE

   Plasmid

   #230910

   Purpose

   Tracing of CRISPR activity in whole animals.

   Depositor

   Michael Boutros

   Article

   Port et al bioRxiv 2024.11.26.625385

   Insert

   GFP
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEE082 FoMV

   Plasmid

   #234811

   Purpose

   To clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA)

   Depositor

   Daniel Voytas

   Article

   Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS159

    Plasmid

    #122378

    Purpose

    CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJPC596 FoMV-AmCyan

    Plasmid

    #234812

    Purpose

    To express flourescent protein AmCyan from the Foxtail Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    AmCyan
    

    Expression

    Plant

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgOpti

    Plasmid

    #85681

    Purpose

    Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

    Depositor

    Eric Lander, David Sabatini

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA-MS2x2-TagRFP657

    Plasmid

    #184441

    Purpose

    sgRNA with MS2 aptamer, TagRFP657 as selection marker

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    sgRNA-MS2x2, TagRFP657
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiGuide Cherry

    Plasmid

    #170510

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Pavel Tolar

    Article

    Newman et al biorXiv 2021.03.02.433398

    Inserts

    S. pyogenes sgRNA cassette
    mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p160Tol2-her4.3:switchCas9

    Plasmid

    #227680

    Purpose

    Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    loxP-dsRed-loxP-Cas9-t2A-GFP
    4xU6:sgRNA
    

    Use

    CRISPR

    Promoter

    U6 and her4.3

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. act5C-mScarlet

    Plasmid

    #230906

    Purpose

    Ubiquitous expression of mScarlet in Drosophila melanogaster

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    mScarlet
    

    Expression

    Insect

    Promoter

    act5C

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRH2521

    Plasmid

    #84380

    Purpose

    Expression of sgRNA from a imyc promoter (Pimyc)

    Depositor

    Robert Husson

    Article

    Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

    Insert

    sgRNA cloning site
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. psPAX2-D64V-NC-COM

    Plasmid

    #131226

    Purpose

    Expressing GAG precursor protein, in which COM is inserted after the second zinc finger domain of nucleocapsid protein (NC)

    Depositor

    Baisong Lu

    Article

    Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

    Insert

    COM
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. gRNA Cloning Vector Bbs I ver. 2

    Plasmid

    #85586

    Purpose

    An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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