We narrowed to 2,322 results for: PUC19

1. pU6_N7-sgRNA2

   Plasmid

   #190664

   Purpose

   Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   N7 sgRNA2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAS12069_U6-pegRNA-mCherry

   Plasmid

   #177180

   Purpose

   pegRNA cloning plasmid with mCherry marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCW01

   Plasmid

   #72300

   Purpose

   Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Type

   Empty backbone

   Use

   Derivative of puc19

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas9

   Plasmid

   #167547

   Purpose

   Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Inserts

   chloramphenicol acetyl transferase
   pUC19 origin of replication
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDAS12222_U6-pegRNA-BFP

   Plasmid

   #177181

   Purpose

   pegRNA cloning plasmid with BFP marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pG108 - K

   Plasmid

   #178039

   Purpose

   Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

   Depositor

   Janina Lewis

   Article

   Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

   Insert

   Kanamycin resistance
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ErmAM has been replaced with Kanamycin for select…

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDAC569

   Plasmid

   #195241

   Purpose

   Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-Lb-crRNA

   Plasmid

   #78957

   Purpose

   Cloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSW039

   Plasmid

   #140035

   Purpose

   KO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoter

   Depositor

   Peter Nixon

   Article

   Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

   Insert

   Yellow fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cLac143

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pG106 - KA

    Plasmid

    #178041

    Purpose

    Bacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markers

    Depositor

    Janina Lewis

    Article

    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

    Type

    Empty backbone

    Use

    Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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