We narrowed to 8,358 results for: BLI;
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Plasmid#104418Purposetransient expression of dCas9-dJMJD2A fusion proteinDepositorInsertdJMJD2A
UseCRISPRTagsExpressionMammalianMutationS288G-T289GPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
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SPSB1
Plasmid#38981PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSPSB1 (SPSB1 Human)
UseTags6xHis-TevExpressionBacterialMutationResidues 24-233 of SPSB1 are present in this plas…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLP-mOlig2-ires-gfp
Plasmid#49399Purposemammalian bicistronic expression of mouse Olig2 and EGFPDepositorInsertOlig2 (Olig2 Mouse)
UseCre/Lox; Acceptor vectorTagsEGFP and IRESExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-CRAF_S257L
Plasmid#51125Purposemammalian expression of wt CRAF S257LDepositorInsertCRAF (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutationS257LPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
PBC002-A04
Plasmid#58761Purposebacterial expression of PDB 4PWYDepositorInsertPDB:4PWY (PEX6 Human)
UseTagsHisExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available sinceAug. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pE-SUMO CRYBB3
Plasmid#80750PurposeExpression of human beta B3-crystallin in E. coliDepositorInsertCRYBB3 (CRYBB3 Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
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PA-TagRFP-Alpha-5-Integrin-12
Plasmid#62791PurposeLocalization: Focal Adhesions, Excitation:N/A, Emission:N/ADepositorInsertAlpha5Integrin (ITGAV Human)
UseTagsTagRFPExpressionMammalianMutationnonePromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pLEX307-CTSL-blast
Plasmid#158467PurposeConstitutive lentiviral expression of human CTSL.DepositorInsertCTSL (CTSL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Enconsin-N-18
Plasmid#62754PurposeLocalization: Microtubule, N1 Cloning Vector, Excitation:487, Emission:509DepositorInsertEnconsin (MAP7 Human)
UseTagsmEmeraldExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFLAG-6a-Ataxin3Q22-C14A
Plasmid#22127DepositorInsertAtaxin 3 (ATXN3 Human)
UseTagsFlagExpressionMammalianMutationC14APromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
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TMPRSS2 g4 + Cas9
Plasmid#153018PurposeA guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2DepositorInsertCas9 + TMPRSS2 gRNA
UseCRISPR and LentiviralTagsTagBFP2ExpressionMutationPromoterAvailable sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1
Plasmid#159910PurposeMutagenesis of Ntsr1DepositorInsertNtsr1 (Ntsr1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pT7-7 asyn S87A/S129A
Plasmid#36063DepositorInsertalpha-synuclein S87A/S129A (SNCA Human)
UseTagsExpressionBacterialMutationS87A/S129APromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-FLAG-hMYF5 (#801)
Plasmid#78334PurposeDoxycycline-inducible lentiviral overexpression of human MYF5 ORF with a FLAG tagDepositorInsertMYF5 (MYF5 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterTRE2Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
KG#865
Plasmid#110912PurposeCoding sequence for attaching the Auxin Inducible Degron to the C-terminus of a protein followed by a 5X Myc tag with spacersDepositorInsertAuxin Inducible Degron-5X Myc-Spacers for C-terminal attachment
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#883
Plasmid#110913PurposeCoding sequence for attaching the a 5X Myc tag with spacers followed by the Auxin Inducible Degron to the N-terminus of a proteinDepositorInsert5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
H11-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP
Plasmid#180157Purposetd-sfGFP dre reporter with Hipp11 recombineering homology armsDepositorInserttd-sfGFP
UseMouse Targeting; Dre/roxTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK650
Plasmid#72400PurposeProduces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)DepositorInsertglutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
UseTagsExpressionBacterialMutationMutated tyrosine-397 to phenylalanine to block ad…PromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK VSV Cortactin
Plasmid#50727Purposemammalian expression of VSV-tagged CortactinDepositorInsertCortactin (CTTN Human)
UseTags2xVSVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-jGCaMP7c variant 1513
Plasmid#173159PurposeExpresses jGCaMP7c variant 1513 in astrocytesDepositorInsertjGCaMP7c variant 1513-WPRE
UseAAVTagsExpressionMutationPromoterGFAPAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-FNLS-T2A-EGFP-ires-puro
Plasmid#121169PurposeCAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.DepositorInsertFNLS-T2A-EGFP-ires-puro
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP
Plasmid#180155Purposetd-sfGFP cre reporter with Hipp11 recombineering homology armsDepositorInserttd-sfGFP
UseCre/Lox and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53205PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and kanMX6ExpressionMutationPromoterGal1 promoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
PTPN5
Plasmid#38887PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPTPN5 (PTPN5 Human)
UseTagsHis TevExpressionBacterialMutationC496S inactivating mutationPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87A/S129E
Plasmid#36065DepositorInsertalpha-synuclein S87A/S129E (SNCA Human)
UseTagsExpressionBacterialMutationS87A/S129EPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKG212
Plasmid#69758PurposeRetroviral expression of CENP-T aa 1-242DepositorInsertCENP-T aa 1-242 (CENPT Human)
UseRetroviralTagsLacI-NLS and mCherryExpressionMammalianMutatione.coli optimized sequencePromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pVP16-tev-PRT1
Plasmid#83405Purposebacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
SDCBPA-c010
Plasmid#175482PurposeProtein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography.DepositorInsertSDCBP (SDCBP Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-6a-Ataxin3Q22-UIM*
Plasmid#22128DepositorInsertAtaxin 3 (ATXN3 Human)
UseTagsFlagExpressionMammalianMutationUIM 1,2,3 SA-DGPromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
CAG-xFNLS-T2A-EGFP-ires-puro
Plasmid#121171PurposeCAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.DepositorInsertxFNLS-T2A-EGFP-ires-puro
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro
Plasmid#121175PurposeDonor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell linesDepositorInsertGCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Cdc42 WT
Plasmid#52248PurposeDrosophila expression of YFP-Cdc42DepositorInsertCdc42 (Cdc42 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only