We narrowed to 12,476 results for: 109

1. TFORF1751

   Plasmid

   #144109

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF180 (ZNF180 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2491

   Plasmid

   #142109

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZBTB17 (ZBTB17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CB6-GFP-TAOK1 (K57A)

   Plasmid

   #197109

   Purpose

   Expression of kinase-deficient GFP-tagged TAOK1 (K57A) / PSK2 (K57A) in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

   Insert

   TAOK1 (K57A) (TAOK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed K 57 to A

   Promoter

   CMV

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-EGFP-C1-HsDCP1a-H19A_V

   Plasmid

   #147799

   Purpose

   Mammalian Expression of HsDCP1a-H19A

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsDCP1a-H19A (DCP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T237C and T1716G compared to…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT7-EGFP-C1-HsDCP1a-N74G_V

   Plasmid

   #147800

   Purpose

   Mammalian Expression of HsDCP1a-N74G

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsDCP1a-N74G (DCP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T237C and T1716G compared to…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7-EGFP-C1-HsDCP1a-Q18AH19A_V

   Plasmid

   #147801

   Purpose

   Mammalian Expression of HsDCP1a-Q18AH19A

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsDCP1a-Q18AH19A (DCP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T237C and T1716G compared to…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT7-EGFP-C1-HsDCP1a-R72G_V

   Plasmid

   #147802

   Purpose

   Mammalian Expression of HsDCP1a-R72G

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsDCP1a-R72G (DCP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T237C and T1716G compared to…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7-EGFP-C1-HsDCP1a-R72GN74G_V

   Plasmid

   #147803

   Purpose

   Mammalian Expression of HsDCP1a-R72GN74G

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsDCP1a-R72GN74G (DCP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T237C and T1716G compared to…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTol2zTagRFP658-EF1-2xCMV-EF1-mClover3

   Plasmid

   #196109

   Purpose

   mammalian/zebrafish expression of zTagRFP658-EF1-2xCMV-EF1-mClover3

   Depositor

   Reinhard Köster

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   zTagRFP658-EF1-2xCMV-EF1-mClover3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-EGFP-C1-HsDCP1a-S12AH19A_V

    Plasmid

    #147804

    Purpose

    Mammalian Expression of HsDCP1a-S12AH19A

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

    Insert

    HsDCP1a-S12AH19A (DCP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations T237C and T1716G compared to…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TetO(3G)-GCaMP6f-WPRE

    Plasmid

    #186205

    Purpose

    Express GCaMP6f under TetO(3G)

    Depositor

    Naoshige Uchida

    Article

    Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mutation

    Promoter

    TetO(3G)

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-EGFP-C1-HsDCP1a-S12AQ18AH19A_V

    Plasmid

    #147805

    Purpose

    Mammalian Expression of HsDCP1a-S12AQ18AH19A

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

    Insert

    HsDCP1a-S12AQ18AH19A (DCP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations T237C and T1716G compared to…

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-Hygro-EF1A-RG-Linker-eGFP

    Plasmid

    #176071

    Purpose

    EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    RG
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLentiCRISPRv2-POLB-KO-g1

    Plasmid

    #176090

    Purpose

    Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    POLB sgRNA #1 (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-4T Doc2beta C2AB(125) H158C

    Plasmid

    #134691

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    H158C, C145A, C217A, C249A, C290A, C338A, C387A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-4T Doc2beta C2AB(125) F222C

    Plasmid

    #134692

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F222C, C145A, C217A, C249A, C290A, C338A, C387A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T Doc2beta C2AB(125) A298C

    Plasmid

    #134693

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    A298C, C145A, C217A, C249A, C290A, C338A, C387A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA4TO-3XFLAG Halo R99A nsp1

    Plasmid

    #175422

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R99A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBlastR-MCS-LexA-VP16-MiniWhite

    Plasmid

    #165900

    Purpose

    Blasticidin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C

    Plasmid

    #81239

    Purpose

    U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studies

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    U6 RNA with A34G/A35G/G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge