We narrowed to 134 results for: spycatcher
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Plasmid#102832PurposeBacterial expression of unreactive version of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002 DA-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationReactive aspartic acid has been mutated to an ala…PromoterT7Available SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC-cUnaG
Plasmid#207631PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.DepositorInsertphotocaged SpyCatcher-cUnaG
Tags6xHisExpressionBacterialMutationAmber stop codon at SC's critical lysinePromoterT7Available SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC-LPETG
Plasmid#207629PurposeA plasmid encoding photocaged SpyCatcher (pSC) with a C-terminal Sortase recognition sequence.DepositorInsertphotocaged SpyCatcher-LPETG
Tags6xHisExpressionBacterialMutationAmber stop codon at SC's critical lysinePromoterT7Available SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
M2e-noro-VLP
Plasmid#201192PurposeExpresses noro-VLP decorated with influenza M2e in insect cellsDepositorInsertM2e-noro-VLP
TagsInfluenza M2e peptideExpressionInsectPromoterPolyhedrinAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-ST-P2A-pSC-CAAX
Plasmid#207637PurposeA plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.DepositorInsertEGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
ExpressionMammalianMutationAmber stop codon at SC's critical lysinePromoterCMVAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC-cUnaG-LPETG
Plasmid#207635PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.DepositorInsertphotocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
Tags6xHisExpressionBacterialMutationAmber stop codon at SC's critical lysinePromoterT7Available SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh
Plasmid#207634PurposeA plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.DepositorInsertphotocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
ExpressionMammalianMutationAmber stop codon at SC's critical lysinePromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-ELP
Plasmid#112630PurposeTwo SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcherDepositorInsertTriCatcher-ELP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-GFP
Plasmid#186900PurposeCell-free expression of transmembrane protein TfR-sfGFP-SpyCatcher-sfCherry1-10DepositorInsertTfR-sfGFP-SpyCatcher003-sfCherry1-10
UseSynthetic BiologyAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only