We narrowed to 128 results for: spycatcher

1. pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh

   Plasmid

   #207634

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE80L TriCatcher-ELP

   Plasmid

   #112630

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-ELP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE80L TriCatcher-RGDSP

   Plasmid

   #112631

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-RGDSP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   central RGDSP integrin-binding sequence

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. InterCatch-GFP

   Plasmid

   #186900

   Purpose

   Cell-free expression of transmembrane protein TfR-sfGFP-SpyCatcher-sfCherry1-10

   Depositor

   Taekjip Ha, Allen Liu

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   TfR-sfGFP-SpyCatcher003-sfCherry1-10
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. sCatch-GFP

   Plasmid

   #186902

   Purpose

   Cell-free expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10

   Depositor

   Taekjip Ha, Allen Liu

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   sfGFP-SpyCatcher003-sfCherry1-10
   

   Use

   Synthetic Biology

   Tags

   sfGFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. sCatch-GFP-6xHIS

   Plasmid

   #186904

   Purpose

   Bacterial expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10 with a 6xHIS tag

   Depositor

   Taekjip Ha, Allen Liu

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   sfGFP-SpyCatcher003-sfCherry1-10-6xHIS
   

   Use

   Tags

   6xHis and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE80L TriCatcher-MMP

   Plasmid

   #112632

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-MMP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   central matrix metalloproteinase-cleavable sequen…

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SpyTag-sfGFP

   Plasmid

   #184226

   Purpose

   Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpyTag-sfGFP
   

   Use

   Tags

   His6, SpyTag, and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE80L TriCatcher-RGDSP-MMP

   Plasmid

   #112633

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-RGDSP-MMP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   central matrix metalloproteinase-cleavable sequen…

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFBD-SpyTag-noro-VLP

    Plasmid

    #165989

    Purpose

    Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyTag-noro-VLP (ORF2 norovirus, Synthetic)
    

    Use

    Tags

    SpyTag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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