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Plasmid#165065PurposeCharacterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMST-1313
Plasmid#118076PurposepTEF1-cexA-tCYC1DepositorInsertpTEF1-cexA-tCYC1
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHBA01
Plasmid#83535PurposeConstitutive TEF1 promoter mediated expression of the UBiC geneDepositorInsertpTEF1-UBiC-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP
Plasmid#137763PurposeControl plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.DepositorInsertGFP
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-DDK
Plasmid#137764PurposeControl plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.DepositorInsertGFP
UseTagsDDKExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-Keima-cb5
Plasmid#137755PurposeTargets Keima to the endoplasmic reticulum. Used to monitor ER-phagyDepositorInsertKeima-cb5
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 W47A-mycDDK
Plasmid#137769PurposeCALCOCO1 LIR mutantDepositorInsertCALCOCO1 W47A
UseTagsmycDDKExpressionMammalianMutationW47APromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-RFP-cb5
Plasmid#137754PurposeTargets red flourescent protein (RFP) to the endoplasmic reticulumDepositorInsertRFP-cb5
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFPU0 (R)
Plasmid#83562PurposeExpress yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (2)
Plasmid#185852PurposeTesting the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP9E3
Plasmid#185842PurposeTesting the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 V142A-mycDDK
Plasmid#137768PurposeCALCOCO1 CLIR mutantDepositorInsertCALCOCO1 V142A
UseTagsmycDDKExpressionMammalianMutationV142APromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEx411c_pelB-NbD6-Strep2
Plasmid#228897PurposePeriplasmic Expresssion of Strep-tagged anti-Zika NbD6DepositorInsertsb0379
UseTagsStrep2ExpressionBacterialMutationPromoterT7Available sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJEx411c_pelB-N32-Strep2
Plasmid#228898PurposePeriplasmic Expresssion of Strep-tagged anti-Zika NbD32DepositorInsertsb0380
UseTagsStrep2ExpressionBacterialMutationPromoterT7Available sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D42
Plasmid#185840PurposeTesting the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (1)
Plasmid#185851PurposeTesting the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 L140A-mycDDK
Plasmid#137767PurposeCALCOCO1 CLIR mutantDepositorInsertCALCOCO1 L140A
UseTagsmycDDKExpressionMammalianMutationL140APromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 R12H-mycDDK
Plasmid#137770PurposeCALCOCO1 R12H mutantDepositorInsertCALCOCO1 R12H
UseTagsmycDDKExpressionMammalianMutationR12HPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFPJ1
Plasmid#83561PurposeExpress yEGFP-ERG9 ER signal peptide under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER signal peptide
UseTagsExpressionYeastMutationPromoterTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPE9
Plasmid#83545PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.DepositorInsertTEF1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd36
Plasmid#98302PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.DepositorInsertPCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIRTetR-GAL80
Plasmid#185853PurposePlugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiaeDepositorInsertgal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#48259PurposeTemplate plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
Ag TEF1 promoter
UseRoutine cloning vectorTagsExpressionMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterAg TEF1 promoterAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pILGFP90
Plasmid#83547PurposeUsed to evaluate the expression output of TEF2 promoter with yEGFP used as the reporter gene.DepositorInsertTEF2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTEF2Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCMM1
Plasmid#167452PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoterDepositorInsertPTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
HA-GABARAP
Plasmid#137760PurposeExpression of GABARAPDepositorInsertGABARAP (GABARAP Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBHM2403
Plasmid#171687PurposepRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.DepositorInsertCnoCAS9
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterTEF1Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1-HA
Plasmid#137766PurposeExpression and localization of tagged CALCOCO1DepositorInsertCALCOCO1 (CALCOCO1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-MAP1LC3C
Plasmid#137758PurposeExpression of LC3CDepositorInsertMAP1LC3C (MAP1LC3C Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only