We narrowed to 14,583 results for: pET

1. pET_3H1L_02.395

   Plasmid

   #155198

   Purpose

   Expresses 3H1L_02.395 in E.coli

   Depositor

   Bruno Correia

   Article

   Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

   Insert

   3H1L_02.395
   

   Use

   Tags

   6x His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETM33_Nsp3c_SUD-C

   Plasmid

   #156471

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3c_SUD-C (ORF1ab Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETM33_Nsp3e_NAB

   Plasmid

   #156473

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET19b_STS:P4S

   Plasmid

   #139797

   Purpose

   expression of biosynthetic enzyme STS in fusion with CC segment P4S

   Depositor

   Roman Jerala

   Article

   Fink et al AMB Express. 2020 May 24;10(1):97. doi:

   Insert

   STS:P4S
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET19b_4CL:P3S

   Plasmid

   #139796

   Purpose

   expression of biosynthetic enzyme 4CL in fusion with CC segment P3S

   Depositor

   Roman Jerala

   Article

   Fink et al AMB Express. 2020 May 24;10(1):97. doi:

   Insert

   4CL:P3S
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET19b_4CL:STS (DF)

   Plasmid

   #139791

   Purpose

   direct fusion of biosynthetic enzymes 4CL and STS

   Depositor

   Roman Jerala

   Article

   Fink et al AMB Express. 2020 May 24;10(1):97. doi:

   Insert

   direct fusion of 4CL and STS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28P_dCrk

   Plasmid

   #131145

   Purpose

   Expresses 6XHis::dCrk (based on crk isoform RA).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21d_SNORD78_probe

   Plasmid

   #73072

   Purpose

   antisense probe for detection of human SNORD78 (C/D box snoRNA U78)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD78 (SNORD78 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pET29b-PD

   Plasmid

   #110168

   Purpose

   Expresses propanediol dehydratase in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Science. 2017 Feb 10;355(6325). pii: 355

   Insert

   Propanediol Dehydratase
   

   Use

   Tags

   His6 and S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET22b_PfSSB_W166C

    Plasmid

    #103001

    Purpose

    The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB (C93A,W166C), used for obtaining a fluorescent adduct at the cysteine

    Depositor

    Martin Webb

    Article

    Chisty et al PLoS One. 2018 Feb 21;13(2):e0193272. do

    Insert

    PfSSB_W166C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C93A, W166C

    Promoter

    T7

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET15T+1X

    Plasmid

    #108188

    Purpose

    PCNA1-PdX fusion protein

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA1-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21a_Msm_RbpA

    Plasmid

    #87928

    Purpose

    Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes

    Depositor

    Seth Darst

    Article

    Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 1

    Insert

    RbpA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7P

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET24a_UDP_Xase_E162

    Plasmid

    #73160

    Purpose

    Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E162A

    Depositor

    Sandra Gabelli

    Article

    Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

    Insert

    pET24a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    change glutamate 162 to alanine

    Promoter

    Available since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pET24_BD3179

    Plasmid

    #73142

    Purpose

    Expresses in E.coli B. bacteriovorus Bd3179

    Depositor

    Sandra Gabelli

    Article

    de la Peña et al PLoS One. 2015 Nov 2;10(11):e0141716. do

    Insert

    Bd3179
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pET11_DHNTPase

    Plasmid

    #73154

    Purpose

    Expresses in E. coli, E. coli Dihydroneopterin triphosphate pyrophosphatase

    Depositor

    Maurice Bessman, Sandra Gabelli

    Article

    Gabelli et al Structure. 2007 Aug;15(8):1014-22.

    Insert

    ORF17_DHNTPase_NUD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pet28a_RBPMS_1_120

    Plasmid

    #65737

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 1-120

    Promoter

    T7

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pet28a_RBPMS_1_144

    Plasmid

    #65739

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 1-144

    Promoter

    T7

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pet23a_RBPMS_20_196

    Plasmid

    #65734

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 20-196

    Promoter

    T7

    Available since

    July 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pet23a_RBPMS_1_129

    Plasmid

    #65731

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 1-129

    Promoter

    T7

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pet23a_RBPMS_1_111

    Plasmid

    #65729

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 1-111

    Promoter

    T7

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pet28a_RBPMS_20_196

    Plasmid

    #65741

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 20-196

    Promoter

    T7

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET:CACT:GFP

    Plasmid

    #60737

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PA1421_WT_pET21a

    Plasmid

    #29792

    Depositor

    SeWon Suh

    Insert

    Guanidinobutyrase (gbuA P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2012

    Availability

    Academic Institutions and Nonprofits only

24. PA0288_WT_pET21a

    Plasmid

    #29793

    Depositor

    SeWon Suh

    Insert

    3-guanidinopropionase (gpuA P. aeruginosa)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2012

    Availability

    Academic Institutions and Nonprofits only

25. PA1421_M161Y_pET21a

    Plasmid

    #29791

    Depositor

    SeWon Suh

    Insert

    Guanidinobutyrase (gbuA P. aeruginosa)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M161Y

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

26. PA0288_Y157M_pET21a

    Plasmid

    #29794

    Depositor

    SeWon Suh

    Insert

    3-guanidinopropionase (gpuA P. aeruginosa)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y157M

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

27. PA RdgC_pET21a

    Plasmid

    #14927

    Depositor

    SeWon Suh

    Article

    Ha et al Nucleic Acids Res. 2007 Apr 10. ():.

    Insert

    Recombination-associated protein rdgC
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pET21a.MJ1228

    Plasmid

    #11410

    Depositor

    Sung-Hou Kim

    Article

    Kim et al Protein Sci. 1997 Oct . 6(10):2268-70.

    Insert

    eukaryotic translation initiation factor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

29. pET21a.AQ_1575

    Plasmid

    #11416

    Depositor

    Sung-Hou Kim

    Article

    Shin et al Proc Natl Acad Sci U S A. 2002 Jun 11. 9

    Insert

    hypothetical (aq_1575 A. aeolicus)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pET28aAviHisCD72aCTLD

    Plasmid

    #129065

    Purpose

    generation of biotinylated mouse CD72a CTLD protein using bacteria

    Depositor

    Takeshi Tsubata

    Article

    Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

    Insert

    CD72a (Cd72 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28aHisAviCD72aCTLD

    Plasmid

    #129067

    Purpose

    generation of biotinylated mouse CD72a CTLD protein using bacteria

    Depositor

    Takeshi Tsubata

    Insert

    CD72a (Cd72 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28aHisAviCD72cCTLD

    Plasmid

    #129068

    Purpose

    generation of biotinylated mouse CD72c CTLD protein using bacteria

    Depositor

    Takeshi Tsubata

    Insert

    CD72c (Cd72 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28aHisAvihCD72CTLD

    Plasmid

    #129069

    Purpose

    generation of biotinylated human CD72 CTLD protein using bacteria

    Depositor

    Takeshi Tsubata

    Insert

    CD72 (CD72 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-NT*-HRV3CP

    Plasmid

    #162795

    Purpose

    Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

    Depositor

    Gottfried Otting

    Article

    Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

    Insert

    Human rhinovirus 14 3C protease
    

    Use

    Tags

    NT* solubility tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET15b-AavLEA1

    Plasmid

    #53093

    Purpose

    AavLEA1 expression (in BL21 DE3 E. coli strain)

    Depositor

    Claude Férec

    Article

    Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

    Insert

    AavLEA1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET29a-YS14

    Plasmid

    #66890

    Purpose

    Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

    Depositor

    Jung-Hyun Min

    Article

    Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

    Inserts

    His6-Thrombin-Xenopus laevis histones H2A
    Xenopus laevis histones H2B
    Xenopus laevis histones H3
    Xenopus laevis histones H4-Thrombin
    

    Use

    Tags

    H2A, H2B, H3, H4, His6 tag, and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pETMCSI-T4P_Y320A

    Plasmid

    #122522

    Purpose

    Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

    Depositor

    Gottfried Otting

    Article

    Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

    Insert

    gp43 - DNA polymerase (43 Escherichia virus T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    tyrosine 320 changed to alanine

    Promoter

    T7 promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pETCON-HtsptLB12v.3

    Plasmid

    #45121

    Depositor

    David Baker

    Article

    Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

    Insert

    HtsptLB12v.3
    

    Use

    Tags

    Aga2p for yeast surface display and c-myc epitope…

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET-Expotin 1

    Plasmid

    #42047

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    Exportin-1 (XPO1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge