We narrowed to 2,361 results for: EFS
-
-
pMito-PAGFP-FRB.
Plasmid#60005PurposeExpresses MitoTrap tagged with photo-activatable GFP in mammalian cells.DepositorTypeEmpty backboneUseKnocksidewaysTagsFRB and PAGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-ΔF3
Plasmid#163618Purposemouse SNX27 without the F3 domain cDNA C-terminally tagged with GFPDepositorInsertmouse SNX27-ΔF3 (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationdeletion F3 domainPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-H112A
Plasmid#163619Purposemouse SNX27 cDNA with a point mutation H112A C-terminally tagged with GFPDepositorInsertmouse SNX27-H112A (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationH112APromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gfap-Cryab
Plasmid#58947Purposehamster Cryab gene under the control of the human GFAP promoter to target expression to astrocytesDepositorInsertCryab
UseMouse Targeting; Transgene expressionTagsExpressionMammalianMutationgenomic fragmentPromoterhuman GFAP promoter (position -2163 to +47)Available sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Atg32 [200-341] 1B
Plasmid#190431PurposeRecombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.DepositorInsertAtg32
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9n(D10A)-Blast
Plasmid#63593PurposeExpresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A, nickase mutantPromoterEFS-NSAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDIV151
Plasmid#226180PurposeExpresses Cas9 in a backbone compatible with K. phaffii.DepositorInsertCas9
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterGAP1Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27 RNAi-resistant
Plasmid#163620Purposemouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFPDepositorInsertmouse SNX27 RNAi-resistant (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationRNAi resistancePromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCF226
Plasmid#121667PurposeEFS-Cas9-wt-P2A-PuroDepositorInsertcas9
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIV272
Plasmid#226183PurposeExpresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEFL
Plasmid#38121DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF-1Available sinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDIV270
Plasmid#226181PurposeExpresses Cas9 and gRNA targeting KU70 gene in K. phaffii.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Smo-YFP-DEST
Plasmid#49099PurposeExpressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorInsertSmo (Smo Mouse)
UseTagsYFP and acceptor peptide (AP)ExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAGG eGFP
Plasmid#107707Purposeexpress eGFP in variety of cell typesDepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGGAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpRY
Plasmid#198544PurposeExpresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSDDepositorInsertSpRY
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5RS-HERCD533
Plasmid#65224PurposeExpression of dominant negative EGFR in mammalian cellsDepositorInsertHERCD533 (EGFR Human)
UseTagsExpressionMammalianMutationdeleted AA 654-1186PromoterCMVAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
ALFY-FYVE 1G
Plasmid#197045PurposeExpression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.DepositorInsertALFY (WDFY3 Human)
UseTags6xHis-GST-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-Squ:mCherry
Plasmid#20163DepositorInsertspaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
HGXPRT
Plasmid#64201PurposeExpression of Plasmodium falciparum HGXPRTDepositorInsertHypoxanthine guanine xanthine phosphoribosyltransferase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-Squ5'3'
Plasmid#20165DepositorInsertspaghetti squash (squ, myosin II RLC) 5' UTR, ORF (without stop codon) and 3' UTR with multiple cloning site downstream (sqh Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
-
Sc++
Plasmid#198545PurposeExpresses human codon-optimized Sc++ and blasticidin resistance: EFS promoter-Sc++-NLS-FLAG-P2A-BSDDepositorInsertSc++
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpG
Plasmid#198543PurposeExpresses human codon-optimized SpG and blasticidin resistance: EFS promoter-SpG-NLS-FLAG-P2A-BSDDepositorInsertSpG
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_CMV_Prss-Halo-KDEL
Plasmid#216256PurposeCMV promoter-regulated ER targeted HaloTag expression vectorDepositorInsertER retained HaloTag
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-H112A RNAi-resistant
Plasmid#163621Purposemouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFPDepositorInsertmouse SNX27-H112A RNAi-resistant (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationH112A and RNAi resistancePromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
myc-Miro2 (lacking TM)
Plasmid#127614PurposeHuman Miro2 with the transmembrane deletedDepositorInsertMiro2 (RHOT2 Human)
UseTagsmycExpressionMammalianMutationTransmembrane deletionPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR
Plasmid#18070DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW
Plasmid#18072DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-CR
Plasmid#18073DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-CR-CT
Plasmid#18074DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-EF1
Plasmid#18081DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-36aa
Plasmid#18082DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1
Plasmid#72266PurposeExpress gene of interest under EF-1 promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
plex_307-ccdB-iRFP713
Plasmid#201140PurposepLEX_307 plasmid with iRFP713 markerDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex_307-ccdB-EYFP
Plasmid#201146PurposepLEX_307 plasmid with EYFP markerDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex_307-ccdB-hygro
Plasmid#201143PurposepLEX_307 plasmid with hygro markerDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex_307-ccdB-blasticidin
Plasmid#201136PurposepLEX_307 plasmid with blasticidin markerDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex_307-ccdB-zeo
Plasmid#201142PurposepLEX_307 plasmid with zeo markerDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEFL KZ HA
Plasmid#38122DepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterEF-1Available sinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
plex_307-ccdB-EBFP2
Plasmid#201137PurposepLEX_307 plasmid with EBFP2 markerDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex_307-ccdB-g418
Plasmid#201141PurposepLEX_307 plasmid with g418 markerDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICPIS-EF1
Plasmid#73355PurposeAdenoX shuttle vector with EF-1 promoterDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMutationPromoterEF1Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL
Plasmid#216253PurposeFRET-based calcium sensor located in the ER lumen, Tet-inducibleDepositorInsertER-targeted D4 second generation cameleon (FRET calcium sensor)
UseLentiviralTagsREAch and mTurquoiseExpressionMutationPromoterTRE3GAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-AsCpf1-Blast
Plasmid#84750PurposeExpresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertAsCpf1
UseCRISPR and LentiviralTagsNLS-3xHA (C terminal on insert)ExpressionMammalianMutationPromoterEFS-NSAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Sstr3-GFP-DEST
Plasmid#49098PurposeExpressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorInsertSstr3 (Sstr3 Mouse)
UseTagsGFP and acceptor peptide (AP)ExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRTH
Plasmid#198541PurposeExpresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRTH
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-LbCpf1-Blast
Plasmid#84751PurposeExpresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertLbCpf1
UseCRISPR and LentiviralTagsNLS-3xHA (C terminal on insert)ExpressionMammalianMutationPromoterEFS-NSAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIRES_EGFP_GA_KCNE1
Plasmid#173160PurposeFor mammalian expression of KCNE1 and creating variantsDepositorInsertKCNE1 (KCNE1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
evoCas9
Plasmid#138558PurposeExpresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSDDepositorInsertevoCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationM495V, Y515N, K526E, R661QPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only
