We narrowed to 22,295 results for: ESC;

1. pBluescript II NLS-MC-EGFP-tdTomato (pBET2)

   Plasmid

   #240411

   Purpose

   This plasmid facilitates the assessment of MMR proficiency in human living cells

   Depositor

   Isao Kuraoka

   Article

   Takedachi et al Mutat Res. 2022 Jan-Jun;824:111779. doi:

   Insert

   NLS-EGFP fusion protein
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. B. adolescentis mRNA toehold switch sensor

   Plasmid

   #110710

   Purpose

   Toehold switch sensor to detect a species specific mRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   B. adolescentis species specific toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. B. adolescentis 16S toehold switch sensor

   Plasmid

   #110700

   Purpose

   Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   B. adolescentis 16S toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE-puro-THAP11-rescue-3xFLAG

   Plasmid

   #36998

   Depositor

   Debu Chakravarti

   Article

   Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

   Insert

   THAP11 (THAP11 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Mutations were generated at codons 273-275 conver…

   Available Since

   May 21, 2012

   Availability

   Academic Institutions and Nonprofits only

5. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

   Plasmid

   #229498

   Purpose

   This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.

   Depositor

   Davide Ruggero

   Article

   Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

   Insert

   MYC 5'Untranslated region + destabilized GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. p23-NES-EsCas13d-msfGFP-NES-Flag

   Plasmid

   #165074

   Purpose

   overexpression of EsCas13d in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   EsCas13d
   

   Expression

   Mammalian

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a 6xHis PreScission SARS-CoV-2 nsp13

   Plasmid

   #159390

   Purpose

   Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

   Depositor

   Tarun Kapoor

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA

   Plasmid

   #232935

   Purpose

   Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   T2A-eGFP-SV40pA with Grb10 homology arms
   

   Use

   Mouse Targeting

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV N-term HA-FLAG puro BRD7 shRescue

   Plasmid

   #41936

   Depositor

   Stephen Elledge

   Article

   Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   BRD7 (BRD7 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   April 1, 2013

   Availability

   Academic Institutions and Nonprofits only

10. Flag-TRIM24-F869D/F979A/N980A shRescue

    Plasmid

    #28145

    Depositor

    Michelle Barton

    Insert

    TRIM24 (TRIM24 Human)
    

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    F869D, F979A, N980A and aa 308 and 309 mutated fo…

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

11. KL060 - pBlueScript II SK-mAID-EGFP CTCF donor

    Plasmid

    #232926

    Purpose

    Donor plasmid for tagging mouse CTCF with mAID-eGFP at the N-terminus. Used with KL061 - PX330-sgRNA006_CTCF sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    mAID-eGFP with mCTCF homology arms
    

    Use

    Mouse Targeting

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC0096 RESCUE truncation (Cas13 delta 892-1095)

    Plasmid

    #130663

    Purpose

    CMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUE

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    dRanCas13b(delta 892-1095)-ADAR2dd
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET His6 prescission LIC cloning vector (HPKS)

    Plasmid

    #29720

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis and Prescission cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pzk384-pmax-PUFc-FseI-RESCUE-SalI-NES

    Plasmid

    #196831

    Purpose

    Express PUFc-RESCUE-S

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    PUFc-RESCUE
    

    Expression

    Mammalian

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor

    Plasmid

    #232936

    Purpose

    Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    T2A-eGFP-SV40pA with Podxl homology arms
    

    Use

    Mouse Targeting

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

    Plasmid

    #207135

    Purpose

    DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

    Plasmid

    #207136

    Purpose

    DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

    Plasmid

    #132410

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dEsCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R295A; H300A; R849A; H854A

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET His6 MBP prescission LIC cloning vector (HMPKS)

    Plasmid

    #29721

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, MBP, and Prescission cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid

    #171385

    Purpose

    Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized minidCas13X.1-RESCUE-S
    

    Expression

    Mammalian

    Promoter

    CMV, U6

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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