Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,673 results for: pcr

Clear keywords
Showing: 201 - 220 of 4673 results

  2. PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11

    Plasmid
    #211942
    Purpose
    dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)
    Depositor
    Evgeny Kvon
    Publication
    Hollingsworth et al Nat Commun. 2025 Jan 6;16(1):409. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Expression
    Mammalian
    Promoter
    HSP68
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    211942-plasmid-map-sequence-id-426466

    Enlarge

  3. All_in_one_CRISPR/Cas9_LacZ

    Plasmid
    #74293
    Purpose
    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening
    Depositor
    Lynne Postovit
    Publication
    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    LacZ-alpha
    Cas9
    mCherry
    Use
    CRISPR
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    Lac promoter, SV40, and T7 promoter
    Available since
    May 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    74293-plasmid-map-sequence-id-140421

    Enlarge

  4. pAc5 HA3-eDHFR-T2A-puro

    Plasmid
    #86395
    Purpose
    for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection
    Depositor
    David Bentley
    Publication
    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HA3 eDHFR T2A puro
    Use
    CRISPR
    Tags
    HA3 eDHFR T2A puro
    Mutation
    R12Y, G67S and Y100I mutations in E coli DHFR
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86395-plasmid-map-sequence-id-170910

    Enlarge

  5. pAc5 HA3-eDHFR-T2A-neo

    Plasmid
    #86396
    Purpose
    for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection
    Depositor
    David Bentley
    Publication
    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HA3 eDHFR T2A neo
    Use
    CRISPR
    Tags
    HA3 eDHFR T2A neo
    Mutation
    R12Y, G67S and Y100I mutations in E coli DHFR
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86396-plasmid-map-sequence-id-170909

    Enlarge

  10. pVITRO1-dV-IgE/κ

    Plasmid
    #61878
    Purpose
    Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin kappa constant region
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61878-plasmid-map-sequence-id-101191

    Enlarge

  11. pOpen-P45

    Plasmid
    #165570
    Purpose
    Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    P45
    Use
    Synthetic Biology
    Available since
    Aug. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165570-plasmid-map-sequence-id-340824

    Enlarge

  12. pVITRO1-dV-IgE/λ

    Plasmid
    #61879
    Purpose
    Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin lambda constant region
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61879-plasmid-map-sequence-id-333601

    Enlarge

  14. pPT-PCR C2

    Plasmid
    #141247
    Purpose
    PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2
    Depositor
    Bogumil Karas
    Publication
    Cochrane et al Algal Research, 2020. 50, 102014
    Insert
    Mitochondrial Genome of Phaeodactylum tricornutum
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, additional minor mutations…
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    141247-plasmid-map-sequence-id-377468

    Enlarge

  16. pPT-PCR C1

    Plasmid
    #141248
    Purpose
    PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1
    Depositor
    Bogumil Karas
    Publication
    Cochrane et al Algal Research, 2020. 50, 102014
    Insert
    Reduced Mitochondrial Genome of Phaeodactylum tricornutum
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, additional minor mutations…
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    141248-plasmid-map-sequence-id-389038

    Enlarge

  19. JI401: Ad5 E1A/Hexon qRT-PCR standard

    Plasmid
    #131753
    Purpose
    Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Inserts
    Ad5 E1A gene fragment
    Ad5 Hexon gene fragment
    Use
    Standard for qrt-pcr
    Promoter
    N/A
    Available since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131753-plasmid-map-sequence-id-314394

    Enlarge

Showing: 201 - 220 of 4673 results