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ABE-RA5.0

Plasmid

#208312

Purpose

Expresses ABE-RA5.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.0-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.1

Plasmid

#208313

Purpose

Expresses ABE-RA5.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.1-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.3

Plasmid

#208314

Purpose

Expresses ABE-RA5.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.3-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.4

Plasmid

#208315

Purpose

Expresses ABE-RA5.4 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.4-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, R47K, P48A, R51L, I76Y, V82S, A106V, D108G,…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.6

Plasmid

#208317

Purpose

Expresses ABE-RA5.6 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.6-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.5

Plasmid

#208316

Purpose

Expresses ABE-RA5.5 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.5-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJT139_GalL_nCDA1-VRERBE3

Plasmid

#145102

Purpose

Expressing base editor nCDA1-VRERBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

nCDA1-VRERBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUDE1093

Plasmid

#204227

Purpose

Episomal yeast plasmid expressing the Ercas12a

Depositor

Jean-Marc Daran

Article

Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

Insert

Eubacterium rectale cas12a (Ercas12a)

Expression

Yeast

Mutation

codon optimized for S. cerevisiae

Promoter

Saccharomyces cerevisiae PGK1

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

Plasmid

#190154

Purpose

Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp3 (Vamp2 Rat)

Use

AAV

Tags

P2AT2A-EGFP-caax

Expression

Mammalian

Mutation

Truncation that includes only rat Vamp3 AA #1-81

Promoter

MBP

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJT171_GalL_A3AΔ178-BE3

Plasmid

#145121

Purpose

Expressing base editor A3AΔ178-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ178-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(1-178AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT170_GalL_A3AΔ182-BE3

Plasmid

#145120

Purpose

Expressing base editor A3AΔ182-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ182-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(1-182AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT169_GalL_A3AΔ186-BE3

Plasmid

#145119

Purpose

Expressing base editor A3AΔ186-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ186-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(1-186AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT122_GalL_cCDA1-VQRBE3

Plasmid

#145087

Purpose

Expressing base editor cCDA1-VQRBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-VQRBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A, D1135V, R1335Q, T1337R)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pVITRO1-HAPPID1

Plasmid

#204626

Purpose

Expresses the human anti-Phl p 7 IgD/λ antibody 102.1F10 (HAPPID1) in mammalian cells. HAPPID1 binds to the grass pollen allergen Phl p 7 with subnanomolar affinity.

Depositor

James McDonnell

Article

Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75

Inserts

HAPPID1 heavy chain
HAPPID1 light chain

Expression

Mammalian

Promoter

mouse elongation factor 1 alpha and rat elongatio…

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pJT140_GalL_cCDA1-VRERBE3

Plasmid

#145103

Purpose

Expressing base editor cCDA1-VRERBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-VRERBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

Promoter

GalL

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT176_GalL_eA3A-BE3

Plasmid

#145125

Purpose

Expressing base editor eA3A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(N57G)-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(N57G); spCas9(D10A)

Promoter

GalL

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT174_GalL_A3A-Y130F-BE3

Plasmid

#145123

Purpose

Expressing base editor A3A-Y130F-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(Y130F)-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(Y130F); spCas9(D10A)

Promoter

GalL

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT173_GalL_A3A-R128A-BE3

Plasmid

#145122

Purpose

Expressing base editor A3A-R128A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(R128A)-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(R128A); spCas9(D10A)

Promoter

GalL

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT175_GalL_A3A(Y130F)Δ186-BE3

Plasmid

#145124

Purpose

Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(Y130F)Δ186-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(Y130F; 1-186AA); spCas9(D10A)

Promoter

GalL

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT113_GalL_cCDA1-xBE3

Plasmid

#145079

Purpose

Expressing base editor cCDA1-xBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-xBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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