We narrowed to 2,303 results for: dap

1. pBSFI-myr-akt-T308A/S473A

   Plasmid

   #49191

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Expression

   Mutation

   T308A; S473A

   Promoter

   T7

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBSFI-myr-akt-T308D/S473D

   Plasmid

   #49192

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Expression

   Mutation

   T308D; S473D

   Promoter

   T7

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac-GST-DAXX-His

   Plasmid

   #175781

   Purpose

   DAXX protein for baculovirus expression system

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Use

   Tags

   GST and TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. FKBP-beta1(617-949)-GFP

   Plasmid

   #100732

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP1B1 (AP1B1 Human)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOD2045-intControl

   Plasmid

   #100005

   Purpose

   MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Inserts

   zif-1 (zif-1 Nematode)
   unc-119
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. EIF4G2_MIF4G_pGEX

   Plasmid

   #135138

   Purpose

   Recombinant expression of EIF4G2 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   EIF4G2 (EIF4G2 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PDZb-beta2(616-951)-mCherry

   Plasmid

   #100736

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Use

   Tags

   PDZb and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYFP-NGAT(A193T,N194Y)

   Plasmid

   #11388

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Changed Alanine 193 to Threonine, and Asparagine …

   Promoter

   Available since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-BE3RA-P2A-Puro

   Plasmid

   #110839

   Purpose

   Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-DAXX-ΔD/E-His

    Plasmid

    #179618

    Purpose

    Express GST-DAXX-ΔDE-His in bacteria

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 424-495

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti ABE7.10-N-AIDmax

    Plasmid

    #157949

    Purpose

    Lentiviral expression of ABE7.10-N-AIDmax

    Depositor

    Dali Li

    Article

    Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Lenti ABE7.10-N-AIDmax
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-beta2(616-951)

    Plasmid

    #100743

    Purpose

    Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Long isoform

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_MAPK3_Pkinase

    Plasmid

    #110023

    Purpose

    Protein expression and purification of MAPK3_Pkinase

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    MAPK3_Pkinase (MAPK3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-DAXX-D/E-His

    Plasmid

    #179619

    Purpose

    Express DAXX D/E region in bacteria

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Use

    Tags

    GST, His, and TEV

    Expression

    Bacterial

    Mutation

    amino acids 414-505 of DAXX

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOD2049-csnControl

    Plasmid

    #100007

    Purpose

    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2048-csnDEG. Expression is controlled by Posm-6 (ciliated sensory neuron specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Inserts

    zif-1 (zif-1 Nematode)
    unc-119
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti AID-BEmax

    Plasmid

    #157950

    Purpose

    Lentiviral expression of AID-BEmax

    Depositor

    Dali Li

    Article

    Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Lenti AID-BEmax
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PLC-TIR-GFP

    Plasmid

    #52736

    Purpose

    Retroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker

    Depositor

    Jonathan Kagan

    Article

    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

    Insert

    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    

    Use

    Retroviral

    Tags

    GFP and PLCd1 Lipid binding

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-85

    Promoter

    Available since

    June 18, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pLenti-BE3-P2A-Puro

    Plasmid

    #110838

    Purpose

    Lentiviral vector for constitutive expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MZB131

    Plasmid

    #217839

    Purpose

    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged AP3D1 adaptin appendage (920-1153) for pulldown assays

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    AP3D1 (AP3D1 Human)
    

    Use

    Tags

    10x His, HaloTag, and SUMO (SMT3)

    Expression

    Bacterial

    Mutation

    Deleted residues 1-919

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MFE23-ZZ

    Plasmid

    #223553

    Purpose

    MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.

    Depositor

    Jean Gariepy

    Article

    Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

    Insert

    pET302-MFE23-ZZ
    

    Use

    Tags

    HA tag and HIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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