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TLCV2-APOBEC1-YTHmut

Plasmid

#178950

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTHmut-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Promoter

tight TRE

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Con/Fon-GCaMP6M

Plasmid

#137119

Purpose

Intersectional viral expression of GCaMP6M in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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yWL268 MCP-GFP

Plasmid

#197052

Purpose

pUBC-NLS-HA-2xMCP-2xGFP

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-2xGFP

Use

Lentiviral

Expression

Mammalian

Promoter

ubc

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pTiGc

Plasmid

#78314

Purpose

Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).

Depositor

Samantha Sampson

Article

Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/

Inserts

TurboFP635
GFP

Use

Synthetic Biology; Mycobacterial replicating vect…

Expression

Bacterial

Promoter

hsp60 and hsp60(ribo)

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#209730

Purpose

Expresses presynaptic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSG5-BZLF1

Plasmid

#72637

Purpose

Mammalian expression vector for Epstein-Barr virus Zta immediate-early transcriptional regulator

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BZLF1

Expression

Mammalian

Mutation

Q163R

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-All-in-one-hCascade

Plasmid

#134919

Purpose

Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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CSII-prEF1a-mCherry-3xNLS

Plasmid

#125262

Purpose

Fluorescent reporter for cell size

Depositor

Jan Skotheim

Article

Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

Insert

mCherry-3xNLS

Use

Lentiviral

Tags

3xNLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC4A1

Plasmid

#132180

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A1 (SLC4A1 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_BIRC6

Plasmid

#197974

Purpose

Entry clone for wt BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Other

Mutation

L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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